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L-Dehydroascorbate efficiently degrades non-thermal plasma-induced hydrogen peroxide. | LitMetric

L-Dehydroascorbate efficiently degrades non-thermal plasma-induced hydrogen peroxide.

Arch Biochem Biophys

Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Showa-Ku, Nagoya, 466-8550, Japan; Center for Low-temperature Plasma Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8603, Japan. Electronic address:

Published: March 2021

AI Article Synopsis

  • Non-thermal plasma (NTP) devices create reactive oxygen and nitrogen species that can promote blood coagulation, wound healing, and selectively kill cancer cells at near-physiological temperatures.
  • The study utilized advanced spectroscopy techniques to measure the levels of reactive species in the presence of biocompatible antioxidants, revealing the significant scavenging effects of l-ascorbate and other agents on various reactive species.
  • Findings highlight the importance of understanding how antioxidants interact with reactive species generated by NTP, suggesting that further research could improve its medical applications.

Article Abstract

Non-thermal plasma (NTP) devices generate reactive oxygen species (ROS) and reactive nitrogen species, such as singlet oxygen (O), superoxide (O), hydroxyl radical (OH), hydrogen peroxide (HO), ozone, and nitric oxide at near-physiological temperature. In preclinical studies, NTP promotes blood coagulation, wound healing with disinfection, and selective killing of cancer cells. Although these biological effects of NTP have been widely explored, the stoichiometric quantitation of ROS in the liquid phase has not been performed in the presence of biocompatible reducing agents, which may modify the final biological effects of NTP. Here, we utilized electron paramagnetic resonance spectroscopy to quantitate OH, using a spin-trapping probe 5,5-dimethyl-1-pyrroline-N-oxide; O, using a fluorescent probe; and O and HO, using luminescent probes, after NTP exposure in the presence of antioxidants. l-ascorbate (Asc) at 50 μM concentration (physiological concentration in serum) significantly scavenged OH, whereas (-)-epigallocatechin gallate (EGCG) and α-tocopherol were also effective at performing scavenging activities at 250 μM concentrations. Asc significantly scavenged O and HO at 100 μM. l-Dehydroascorbate (DHA), an oxidized form of Asc, degraded HO, whereas it did not quench OH or O, which are sources of HO. Furthermore, EGCG efficiently scavenged NTP-induced O, O, and HO in Chelex-treated water. These results indicate that the redox cycling of Asc/DHA and metabolites of DHA are important to be considered when applying NTP to cells and tissues. Additionally, ROS-reducing compounds, such as EGCG, affect the outcome. Further studies are warranted to elucidate the interaction between ROS and biomolecules to promote the medical applications of NTP.

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Source
http://dx.doi.org/10.1016/j.abb.2021.108762DOI Listing

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