Case: A 19-year-old woman with persistent anterior knee pain was diagnosed with a complex tibial and femoral torsional deformity (26° of femoral anteversion and 49° of tibial external rotation). To achieve the correct realignment of the lower limb, rotational double osteotomies were needed. After planning the correction on the computed tomography scan and three-dimensional (3D) model, a custom-made 3D-printed guiding system was produced to support the surgery.
Conclusion: The 3D-printed planning model and the surgical guiding system are crucial elements to achieve optimal results for complex malalignment cases. The "tailored" guides led to a perfect match between the planned correction and the intraoperative result.
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http://dx.doi.org/10.2106/JBJS.CC.20.00468 | DOI Listing |
J Med Imaging Radiat Oncol
December 2024
Department of Radiology, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia.
Characterisation of an indeterminate ovarian mass is important as it guides management and clinical outcomes. Ultrasound is the first-line modality in the assessment of ovarian tumours. When ovarian masses are indeterminate on ultrasound, MRI provides excellent resolution in tissue characterisation and enhancement patterns.
View Article and Find Full Text PDFMethods Mol Biol
December 2024
Sanya Institute of Nanjing Agricultural University, Department of Plant Pathology, Nanjing Agricultural University, Nanjing, Jiangsu, China.
The establishment of reliable and efficient systems for genome editing in Phytophthora is very important for studying gene functions. Here, step-by-step methods for CRISPR/Cas9-based gene knockout and in situ complementation for Phytophthora sojae are presented. These steps include the sgRNA design, Cas9-sgRNA plasmid construction, homologous replacement, complementation vector construction, P.
View Article and Find Full Text PDFMethods Mol Biol
December 2024
Department of Microbiology and Plant Pathology, University of California, Riverside, CA, USA.
Molecular genetic tools such as CRISPR-Cas gene editing systems are invaluable for understanding gene and protein function and revealing the details of a pathogen's life and disease cycles. Here we present protocols for genome editing in Phytophthora infestans, an oomycete with global importance as a pathogen of potato and tomato. Using a vector system that expresses variants of Cas12a from Lachnospiraceae bacterium and its guide RNA from a unified transcript, we first present a method for editing genes through the non-homologous end-joining (NHEJ) pathway.
View Article and Find Full Text PDF3D Print Med
December 2024
Diagnostic Imaging Department, Hospital de la Santa Creu i Sant Pau, Sant Antoni Maria Claret 167, 08025, Barcelona, Spain.
Background: 3D technologies [Virtual and Augmented 3D planning, 3D printing (3DP), Additive Manufacturing (AM)] are rapidly being adopted in the healthcare sector, demonstrating their relevance in personalized medicine and the rapid development of medical devices. The study's purpose was to understand the state and evolution of 3DP/AM technologies at the Point-of-Care (PoC), its adoption, organization and process in Spanish hospitals and to understand and compare the evolution of the models, clinical applications, and challenges in utilizing the technology during the COVID-19 pandemic and beyond.
Methods: This was a questionnaire-based qualitative and longitudinal study.
Tomography
December 2024
Department of Radiology, University of Massachusetts Medical Center, Worcester, MA 01655, USA.
Objective: Image-guided diagnosis and treatment of lung lesions is an active area of research. With the growing number of solutions proposed, there is also a growing need to establish a standard for the evaluation of these solutions. Thus, realistic phantom and preclinical environments must be established.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!