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Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus. | LitMetric

AI Article Synopsis

  • Rhodnius prolixus is a significant vector for Chagas disease, and recent research sequenced its genome, improving gene annotations for 9,206 genes.
  • This study involved analyzing around 228 million RNA-Seq reads, which identified over 2,000 new genes and increased the total gene count from 15,738 to 17,864.
  • The findings reveal key gene expression changes during oogenesis, highlighting crucial factors for germline development and early embryonic processes, particularly in mature eggs where various signaling pathways are active.

Article Abstract

Rhodnius prolixus is a Triatominae insect species and a primary vector of Chagas disease. The genome of R. prolixus has been recently sequenced and partially assembled, but few transcriptome analyses have been performed to date. In this study, we describe the stage-specific transcriptomes obtained from previtellogenic stages of oogenesis and from mature eggs. By analyzing ~ 228 million paired-end RNA-Seq reads, we significantly improved the current genome annotations for 9206 genes. We provide extended 5' and 3' UTRs, complete Open Reading Frames, and alternative transcript variants. Strikingly, using a combination of genome-guided and de novo transcriptome assembly we found more than two thousand novel genes, thus increasing the number of genes in R. prolixus from 15,738 to 17,864. We used the improved transcriptome to investigate stage-specific gene expression profiles during R. prolixus oogenesis. Our data reveal that 11,127 genes are expressed in the early previtellogenic stage of oogenesis and their transcripts are deposited in the developing egg including key factors regulating germline development, genome integrity, and the maternal-zygotic transition. In addition, GO term analyses show that transcripts encoding components of the steroid hormone receptor pathway, cytoskeleton, and intracellular signaling are abundant in the mature eggs, where they likely control early embryonic development upon fertilization. Our results significantly improve the R. prolixus genome and transcriptome and provide novel insight into oogenesis and early embryogenesis in this medically relevant insect.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7820597PMC
http://dx.doi.org/10.1038/s41598-021-81387-1DOI Listing

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