Improved spectral resolution for the rapid simultaneous spectrophotometric determination of sofosbuvir and daclatasvir as anti hepatitis C virus drugs in pharmaceutical formulation and biological fluid using continuous wavelet and derivative transform.

In this study, the simultaneous spectrophotometric estimation of Sofosbuvir (SOF) and Daclatasvir (DAC) in synthetic mixtures and tablet formulation in the presence of overlapping spectra was performed based on continuous wavelet transform (CWT) and derivative spectrophotometry (DS) methods without any separation process. The Coiflet (Coif2) and Daubechies (Db3) wavelet families with wavelength of 256 nm and 218 nm were obtained as the best families for the simultaneous determination of SOF and DAC, respectively. Also, the first derivative absorption spectra revealed the best results corresponding to the analysis of SOF and DAC at 237 nm and 291 nm, respectively. The ranges of limit of detection (LOD) and limit of quantitation (LOQ) related to the CWT and DS methods were 2.45 × 10 to 0.5054 and 6.91 × 10 to 0.6027, respectively. Mean recovery values of SOF and DAC in synthetic mixtures for CWT approach were 98.55%, 98.09% and in DS method were 98.78% and 95.83%, respectively. Real samples, including Sovodak tablet and urine was used for accurate simultaneous determination of the mentioned components. Analyzing Sovodak tablet was implemented using high-performance liquid chromatography (HPLC) as a reference method that the results were near to the CWT and DS methods. In order to investigate the existence of significant differences between the methods, analysis of variance (ANOVA) test at the 95% confidence level was performed but no significant differences were observed. In addition, the amounts of SOF and DAC in the complex matrix of biological sample were well predicted by the proposed methods.