AI Article Synopsis
- The study examines the effectiveness of Purexa™-MQ anion-exchange membranes for protein polishing amid high solution conductivities, specifically evaluating dynamic binding capacities (DBC) for various proteins and DNA.
- Results show that Purexa™-MQ achieves a high DBC of over 90 mg/ml for bovine serum albumin (BSA) at conductivities up to 15 mS/cm and maintains consistent performance across multiple bind-elute cycles.
- Additionally, Purexa™-MQ demonstrates superior clearance of host cell proteins and aggregates in monoclonal antibodies compared to other media, and exhibits a strong DBC for salmon sperm DNA, suggesting potential for use in plasmid DNA purification.
Article Abstract
This contribution reports on a study using Purexa™-MQ multimodal anion-exchange (AEX) membranes for protein polishing at elevated solution conductivities. Dynamic binding capacities (DBC ) of bovine serum albumin (BSA), human immunoglobulins, and salmon sperm DNA (ss-DNA) are reported for various salt types, salt concentrations, flowrates, and pH. Using 1 mg/ml BSA, DBC values for Purexa™-MQ were >90 mg/ml at conductivities up to 15 mS/cm. The membranes maintained a high, salt-tolerant BSA DBC of 89.8 ± 2.7 (SD) over the course of 100 bind-elute cycles. Polishing studies with acidic and basic monoclonal antibodies at >2 kg/L loads showed that Purexa™-MQ had higher clearance of host cell proteins and aggregate species at high conductivity (13 mS/cm) and in the presence of phosphate than other commercial AEX media. Purexa™-MQ also had a high ss-DNA DBC of 50 mg/ml at conductivities up to 15 mS/cm, markedly outperforming other commercial products. In addition to the effectiveness of Purexa™-MQ for protein polishing at elevated solution conductivities, its unusually high binding capacity for ss-DNA indicates potential applications for plasmid DNA purification.
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| http://dx.doi.org/10.1002/btpr.3129 | DOI Listing |
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