AI Article Synopsis
- The study aimed to genetically analyze Giardia isolates from patients in the Chandigarh region of India using nested PCR targeting the GLUD1 gene.
- Out of 40 tested samples, the GLUD1 gene was amplified in 33, with successful sequencing in 32.
- Most isolates belonged to assemblage B (84.37%), primarily of subtype BIII, suggesting that genotyping can aid epidemiological studies of Giardia.
Article Abstract
Objective: The aim of study was to characterize Giardia isolates genetically among patients in Chandigarh region, India. For this, nested PCR targeting fragment of the glutamate dehydrogenase (GLUD1 earlier named as GDH) gene was used. Phylogenetic analysis was done by constructing neighbor-joining tree made out of the nucleotide sequences of G. intestinalis isolates obtained in this study and with the known sequences published in GenBank.
Results: Out of 40 samples, GLUD1 gene was amplified in 33 samples (82.5%). The product of GLUD1 gene was successfully sequenced only in 32 samples. In these samples, assemblage B was found in 27 (84.37%) samples whereas 5 (15.6%) samples had assemblage A. Among assemblage B most of them were of BIII. Therefore, genotyping of Giardia would be helpful in conducting epidemiological studies.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816347 | PMC |
| http://dx.doi.org/10.1186/s13104-020-05419-1 | DOI Listing |
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