AI Article Synopsis
- A four-antigen vaccine (SA4Ag) was developed to protect surgical patients from invasive infections, incorporating various components like polysaccharide conjugates and mutant proteins.
- The vaccine was tested in several mouse and rat models to measure its effectiveness against different stages of invasive infection, showing a significant reduction in bacterial load in most models, including complete prevention in an endocarditis model.
- Despite promising preclinical results, the SA4Ag vaccine did not demonstrate its clinical effectiveness in preventing invasive infections associated with surgery.
Article Abstract
A four-antigen vaccine (SA4Ag) was designed for the prevention of invasive disease in surgical patients. The vaccine is composed of capsular polysaccharide type 5 and type 8 CRM conjugates, a clumping factor A mutant (Y338A-ClfA) and manganese transporter subunit C (MntC). pathogenicity is characterized by an ability to rapidly adapt to the host environment during infection, which can progress from a local infection to sepsis and invasion of distant organs. To test the protective capacity of the SA4Ag vaccine against progressive disease stages of an invasive infection, a deep tissue infection mouse model, a bacteremia mouse model, a pyelonephritis model, and a rat model of infectious endocarditis were utilized. SA4Ag vaccination significantly reduced the bacterial burden in deep tissue infection, in bacteremia, and in the pyelonephritis model. Complete prevention of infection was demonstrated in a clinically relevant endocarditis model. Unfortunately, these positive preclinical findings with SA4Ag did not prove the clinical utility of SA4Ag in the prevention of surgery-associated invasive infection.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830931 | PMC |
| http://dx.doi.org/10.3390/microorganisms9010177 | DOI Listing |
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