Laminin is a basement membrane-specific glycoprotein that promotes cell adhesion, proliferation, differentiation, and tumor cell migration. Synthetic peptides from the amino acid sequence deduced from a cDNA clone of the B1 chain of laminin were tested for their ability to promote the migration of B16F10 melanoma cells. A peptide, CDPGYIGSR, that is able to mediate epithelial cell attachment to laminin was found to promote migration, and the constituent pentapeptide YIGSR was also active but to a lesser degree. This nine-amino acid peptide blocked migration of melanoma cells to laminin but had no effect on migration to fibronectin. These data suggest that the cell-binding site and migration site on laminin share a common sequence that is unique to laminin.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jcp.1041340216 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ribitol and ribose treatments differentially affect metabolism of muscle tissue in FKRP mutant mice.
Sci Rep
January 2025
McColl-Lockwood Laboratory for Muscular Dystrophy Research, Carolinas Medical Center, Atrium Health Musculoskeletal Institute, 1000 Blythe Blvd. , Charlotte, NC, 28231, USA.
Dystroglycanopathy is characterized by reduced or lack of matriglycan, a cellular receptor for laminin as well as other extracellular matrix proteins. Recent studies have delineated the glycan chain structure of the matriglycan and the pathway with key components identified. FKRP functions as ribitol-5-phosphate transferase with CDP-ribitol as the substrate for the extension of the glycan chain.
View Article and Find Full Text PDFIn Vivo-Like Scaffold-Free 3D In Vitro Models of Muscular Dystrophies: The Case for Anchored Cell Sheet Engineering in Personalized Medicine.
Adv Healthc Mater
December 2024
Evolved.Bio, 280 Joseph Street, Kitchener, Ontario, N2G4Z5, Canada.
Progress in understanding the underlying mechanisms of muscular dystrophies is hindered by the lack of pathophysiologically relevant in vitro models. Here, an entirely scaffold-free anchored cell sheet engineering platform is used to create patient-specific three-dimensional (3D) skeletal muscle in vitro models. This approach effectively replicates mature muscle phenotypes and tissue- and disease-specific extracellular matric (ECM).
View Article and Find Full Text PDFIntegrating bioinformatics and machine learning to unravel shared mechanisms and biomarkers in chronic obstructive pulmonary disease and type 2 diabetes.
Postgrad Med J
December 2024
Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Anhui Medical University, Jixi Road 218, Hefei 230022, China.
Background: Chronic obstructive pulmonary disease (COPD) and type 2 diabetes mellitus (T2DM) are on the rise. While there is evidence of a link between the two diseases, the pathophysiological mechanisms they share are not fully understood.
Methods: In this study, the co-expressed genes of COPD and T2DM in Gene Expression Omnibus database were identified by bioinformatics method, and the functional enrichment analysis was performed.
A new insight on alleviating the inhibitory effect of aflatoxin B1 on muscle development in grass carp (): The effect of 4-Methylesculetin in vivo and in vitro.
Anim Nutr
December 2024
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China.
Aflatoxin B1 (AFB1), an important fungal toxin, exists mainly in plant feed ingredients and animals consuming feed contaminated with AFB1 will have reduced growth and impaired health condition mainly due to oxidative stress and reduced immunity. Our previous study found that AFB1 caused oxidative damage and inhibited muscle development of zebrafish. 4-Methylesculetin (4-ME), a coumarin derivative, is now used in biochemistry and medicine widely because of its antioxidant function.
View Article and Find Full Text PDFMirror-polished ceria-stabilized zirconia/alumina nanocomposite enhances gingival junctional epithelial cell adhesion.
J Oral Biosci
December 2024
Department of Prosthodontics, Showa University School of Dentistry, Tokyo, Japan. Electronic address:
Objectives: The aim of this study was to determine the optimal surface roughness (Ra) of ceria-stabilized zirconia/alumina nanocomposite (Ce-TZP/Al₂O₃) implants for mouse gingival junctional epithelial cell (JE-1) adhesion and soft tissue sealing in vitro.
Methods: Titanium and Ce-TZP/Al₂O₃ disks were prepared, mechanically polished (M), and mirror-polished (Mr). The surface morphology of each disk was evaluated, and the Ra was measured using scanning electron microscopy and atomic force microscopy.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!