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Application of Lithium Assay kit LS for quantification of lithium in whole blood and urine. | LitMetric

Application of Lithium Assay kit LS for quantification of lithium in whole blood and urine.

Leg Med (Tokyo)

Department of Legal Medicine, Aichi Medical University School of Medicine, 1-1 Yazakokarimata, Nagakute, Aichi 480-1195, Japan; Poison Analysis Center, Aichi Medical University School of Medicine, 1-1 Yazakokarimata, Nagakute, Aichi 480-1195, Japan.

Published: March 2021

AI Article Synopsis

  • A study explored the use of the Lithium Assay kit LS, originally designed for serum or plasma, to measure lithium levels in whole blood and urine from autopsy samples.
  • The kit exhibited strong performance in whole blood (0.5-20 µg/mL range) after sample pretreatment, and excellent accuracy in unprocessed urine.
  • Results showed satisfactory measurements in higher lithium concentrations, making the kit a promising tool for forensic toxicology due to its straightforward and rapid application.

Article Abstract

A commercially available kit for the quantitation of lithium, the Lithium Assay kit LS, was originally developed to measure lithium in serum or plasma using a conventional microplate reader. We investigated whether use of the kit could be extended to quantify lithium in whole blood and urine samples collected at autopsy. The calibration curve for whole blood showed good linearity ranging from 0.5 to 20 µg/mL with a coefficient of determination of 0.998 when samples were pretreated with methanol followed by acetonitrile. Moreover, for urine, we obtained excellent linearity with a coefficient of determination of 0.999 without any pretreatment. The accuracies and precisions were 106.3-174.7% and 1.9-18.1% for whole blood and 83.3-118.8% and 5.7-33.8% for urine. The values in the lower concentration range (0.5-1 µg/mL) were not satisfactory, whereas those in the higher range (2-20 µg/mL) were acceptable. The Lithium Assay kit LS was successfully applied to the measurement of lithium in whole blood and urine samples collected at autopsies. This method appears to be useful for forensic toxicological investigations because of its simplicity and speed.

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Source
http://dx.doi.org/10.1016/j.legalmed.2020.101834DOI Listing

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