Characterization of () homologue gene in WSP-V2 and its regulation by the biocontrol agent OKC.

() is a protein kinase and plays an important role in the energy homeostasis of glucose repressible gene transcription. It derepresses glucose repressed genes and associated with pathogenesis and production of cell wall degrading enzymes in fungal species. In the present study, we identified and characterized homologue in the strain WSP-V2. Transcript analysis of along with the MAP kinases and some cell wall degrading enzyme (CWDE) genes of during interaction with pigeonpea revealed that most MAP kinases and CWDE genes was positively correlated with the gene. Interestingly, transcript accumulation of all these genes was lowered when pigeonpea seeds were bioprimed with a PGPR strain OKC. Transcript accumulation of was observed from the day of inoculation and reached maximum level on day 7 in OKC non-bioprimed plants. However, transcript accumulation was low (1.5 fold) in inoculated with pigeonpea plants bioprimed with OKC. Transcript accumulation patterns of the MAP Kinases genes and CWDE genes also showed a similar trend and their transcript accumulation was lowered in the OKC bioprimed treatment. The results thus indicate a prime role of in regulating pathogenicity and virulence of The results further emphasize the importance of application of effective PGPR strains in regulating virulence of In silico analysis of the SNF1 reference proteins from different fungal species showed that their homologue FuSNF1 is likely to be thermostable and acidic in nature.