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Transport of environmental natural organic matter coated silver nanoparticle across cell membrane based on membrane etching treatment and inhibitors. | LitMetric

Transport of environmental natural organic matter coated silver nanoparticle across cell membrane based on membrane etching treatment and inhibitors.

Sci Rep

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering and Centre of Materials Analysis, Nanjing University, Nanjing, 210023, People's Republic of China.

Published: January 2021

AI Article Synopsis

  • Environmental natural organic matters (NOMs) significantly affect the behavior and toxicity of engineering nanoparticles, particularly silver nanoparticles (AgNPs).
  • The study examines how different coatings like citric acid (CA), tartaric acid (TA), and fulvic acid (FA) influence the stability, uptake, and transport of AgNPs into A549 cells.
  • Results indicate that NOMs enhance AgNP colloidal stability, increase the rate of cellular uptake compared to uncoated AgNPs, and alter the mechanisms of uptake, demonstrating that NOMs-coated AgNPs behave differently from uncoated ones.

Article Abstract

Environmental natural organic matters (NOMs) have great effects on the physicochemical properties of engineering nanoparticles, which may impact the transport of nanoparticles across plasma membrane and the cytotoxicity. Therefore, the kinetics, uptake pathway and mass of transporting into A549 cell membrane of silver nanoparticles (AgNPs) coated with citric acid (CA), tartaric acid (TA) and fulvic acid (FA) were investigated, respectively. CA, FA and TA enhanced the colloidal stability of AgNPs in culture medium and have greatly changed the surface plasmon resonance spectrum of AgNPs due to the absorption of CA, FA and TA on surface of AgNPs. Internalizing model showed that velocity of CA-, TA- and FA-nAg transporting into A549 cell were 5.82-, 1.69- and 0.29-fold higher than those of the control group, respectively. Intracellular mass of Ag was dependent on mass of AgNPs delivered to cell from suspension, which obeyed Logistic model and was affected by NOMs that CA- and TA-nAg showed a large promotion on intracellular mass of Ag. The lipid raft/caveolae-mediated endocytosis (LME) of A549 cell uptake of AgNPs were susceptible to CA, TA and FA that uptake of CA-, TA- and FA-nAg showed lower degree of dependent on LME than that of the control (uncoated AgNPs). Actin-involved uptake pathway and macropinocytosis would have less contribution to uptake of FA-nAg. Overall, transmembrane transport of NOMs-coated AgNPs differs greatly from that of the pristine AgNPs.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7803783PMC
http://dx.doi.org/10.1038/s41598-020-79901-yDOI Listing

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