AI Article Synopsis
- Jacob is a protein that helps link NMDAR activity (related to neurotransmitters) to gene expression controlled by CREB, a transcription factor.
- The study found that Jacob is mainly located at the nuclear envelope and its positioning depends on NMDAR activity and its transport into the nucleus.
- Jacob temporarily associates with the inner nuclear membrane by binding to LaminB1 and utilizes a specific export mechanism for its movement, indicating a process of relocation within the nucleus after it enters and before it exits.
Article Abstract
Jacob is a synapto-nuclear messenger protein that couples NMDAR activity to CREB-dependent gene expression. In this study, we investigated the nuclear distribution of Jacob and report a prominent targeting to the nuclear envelope that requires NMDAR activity and nuclear import. Immunogold electron microscopy and proximity ligation assay combined with STED imaging revealed preferential association of Jacob with the inner nuclear membrane where it directly binds to LaminB1, an intermediate filament and core component of the inner nuclear membrane (INM). The association with the INM is transient; it involves a functional nuclear export signal in Jacob and a canonical CRM1-RanGTP-dependent export mechanism that defines the residing time of the protein at the INM. Taken together, the data suggest a stepwise redistribution of Jacob within the nucleus following nuclear import and prior to nuclear export.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7802242 | PMC |
| http://dx.doi.org/10.1186/s13041-020-00722-1 | DOI Listing |
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