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Overexpression of the Bacteriophage T4 Gene Alters H-NS Dependent Repression of Specific Host DNA. | LitMetric

Overexpression of the Bacteriophage T4 Gene Alters H-NS Dependent Repression of Specific Host DNA.

Viruses

Gene Expression and Regulation Section, Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Published: January 2021

AI Article Synopsis

  • The T4 bacteriophage protein MotB binds to DNA and interacts with the host protein H-NS, enhancing the phage's fitness without significantly altering the T4 transcriptome when knocked down.
  • MotB is evolutionarily conserved, featuring a predicted structure that consists of a KOW motif and an OB-fold DNA-binding domain, suggesting its capability to bind DNA.
  • RNA-seq analyses reveal that overexpression of MotB up-regulates 75 host genes, mainly linked to the H-NS regulon, potentially altering host DNA interactions to create conditions favorable for T4 infection.

Article Abstract

The bacteriophage T4 early gene product MotB binds tightly but nonspecifically to DNA, copurifies with the host Nucleoid Associated Protein (NAP) H-NS in the presence of DNA and improves T4 fitness. However, the T4 transcriptome is not significantly affected by a knockdown. Here we have investigated the phylogeny of MotB and its predicted domains, how MotB and H-NS together interact with DNA, and how heterologous overexpression of impacts host gene expression. We find that is highly conserved among . Although the MotB sequence has no homology to proteins of known function, predicted structure homology searches suggest that MotB is composed of an N-terminal Kyprides-Onzonis-Woese (KOW) motif and a C-terminal DNA-binding domain of oligonucleotide/oligosaccharide (OB)-fold; either of which could provide MotB's ability to bind DNA. DNase I footprinting demonstrates that MotB dramatically alters the interaction of H-NS with DNA in vitro. RNA-seq analyses indicate that expression of plasmid-borne up-regulates 75 host genes; no host genes are down-regulated. Approximately 1/3 of the up-regulated genes have previously been shown to be part of the H-NS regulon. Our results indicate that MotB provides a conserved function for and suggest a model in which MotB functions to alter the host transcriptome, possibly by changing the association of H-NS with the host DNA, which then leads to conditions that are more favorable for infection.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7827196PMC
http://dx.doi.org/10.3390/v13010084DOI Listing

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