Pathogenicity and Side Effect of Indigenous on and (Coleoptera: Coccinellidae).

This study aimed to estimate the virulence of an indigenous on all developmental stages of two indigenous coccinellids; and through three application methods; direct spray, contact method, and feeding on aphids treated with the fungus (ingestion). Also, indirect effect on all developmental stages resulted from 1st larval instar treated with these application methods. All treatments were done with a concentration of 1 × 10 which was recommended in previous studies for different aphid species with a control of 0.02% Tween 80 (/). The mortality of 1st larval instar of both and and pupal stage of were significantly increased with spray method only. Also, contact method achieved significantly higher mortality on 1st larval instar of only. Regard to indirect effect, except of mortality of 1st larval instar of both predators and 2nd larval instar of other developmental instars/stages of both predators were not affected by through the three tested application methods in the mortality, duration, survival, cumulative survival male and female longevity, and fecundity. Therefore, both tested predatory coccinellids could be compatible with this indigenous isolate of where, in general, there are no negative effects of the fungus on both predators.