dmrt11E ortholog is a crucial factor for oogenesis of the domesticated silkworm, Bombyx mori.

DMRT (Doublesex and Mab-3-related transcription factor) is a highly conserved transcription factor family involved in sex determination in numerous animal species. One DMRT, dmrt2/dmrt11E, has entirely different functions in invertebrate and vertebrate species, indicating unpredicted functions. Here, we performed functional analysis of the dmrt11E gene in the domesticated silkworm, Bombyx mori. This gene was preferentially expressed in ovarioles at the last larval instar stage. Its mRNA accumulated in ovarian eggs during the adult stage. CRISPR/Cas9-mediated knockout of Bombyx dmrt11E (Bmdmrt11E) caused defects in oogenesis, resulting in the production of abnormal eggs with transparent liquids. These eggs had significantly reduced fertility and lipid levels. Transcriptomic comparisons between ovaries of control and mutant insects at two developmental stages identified six genes that may be under the control of Bmdmrt11E. Finally, we provide a possible model for lipid uptake and storage in eggs of Bombyx mori.