AI Article Synopsis
- The research focuses on improving squalene production in the yeast Yarrowia lipolytica through genetic and process engineering techniques.
- The team initially overexpressed HMG-CoA reductase, leading to a production of 208.88 mg/L squalene, and then achieved a higher production of 439.14 mg/L by also co-overexpressing diacylglycerol acyltransferase.
- After optimizing the fermentation medium and adding specific compounds, the best yield reached an impressive 731.18 mg/L, marking the highest squalene production reported in this yeast and laying groundwork for more efficient production methods.
Article Abstract
The aim of this present research is to enhance the squalene production in Yarrowia lipolytica using pathway engineering and bioprocess engineering. Firstly, to improve the production of squalene, the endogenous HMG-CoA reductase (HMG1) was overexpressed in Y. lipolytica to yield 208.88 mg/L squalene. Secondly, the HMG1 and diacylglycerol acyltranferase (DGA1) were co-overexpressed, the derived recombinant Y. lipolytica SQ-1 strain produced 439.14 mg/L of squalene. Thirdly, by optimizing the fermentation medium, the improved titer of squalene with 514.34 mg/L was obtained by the engineered strain SQ-1 grown on YPD-80 medium. Finally, by optimizing the addition concentrations of acetate, citrate and terbinafine, the 731.18 mg/L squalene was produced in the engineered strain SQ-1 with the addition of 0.5 mg/L terbinafine. This work describes the highest reported squalene titer in Y. lipolytica to date. This study will provide the foundation for further engineering Y. lipolytica capable of cost-efficiently producing squalene.
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| http://dx.doi.org/10.1016/j.biortech.2020.124652 | DOI Listing |
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