AI Article Synopsis
- Toxic and volatile chemicals like acrolein, found in household products and used in warfare, pose significant public health risks.
- This study investigates how acrolein harms the cornea by assessing human corneal stromal fibroblasts (hCSFs) and exploring protective measures with N-acetylcysteine (NAC).
- Findings revealed that acrolein exposure increases reactive oxygen species (ROS), reduces glutathione (GSH) levels, and causes mitochondrial dysfunction leading to cell death, but these effects can be reduced with NAC treatment.
Article Abstract
Toxic and volatile chemicals are widely used in household products and previously used as warfare agents, causing a public health threat worldwide. This study aimed to evaluate the extent of injury and mechanisms of acrolein toxicity in the cornea. Primary human corneal stromal fibroblasts cultures (hCSFs) from human donor cornea were cultured and exposed to acrolein toxicity with -/+ N-acetylcysteine (NAC) to study the mode of action in the presence of Buthionine sulphoximine (BSO). PrestoBlue and MTT assays were used to optimize acrolein, NAC, and BSO doses for hCSFs. Cell-based assays and qRT-PCR analyses were performed to understand the acrolein toxicity and mechanisms. Acrolein exposure leads to an increased reactive oxygen species (ROS), compromised glutathione (GSH) levels, and mitochondrial dysfunction. The TUNEL and caspase assays showed that acrolein caused cell death in hCSFs. These deleterious effects can be mitigated using NAC in hCSFs, suggesting that GSH can be a potential target for acrolein toxicity in the cornea.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206442 | PMC |
| http://dx.doi.org/10.1016/j.toxlet.2021.01.005 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!