The bioactive chemicals in L. cuneata were investigated by repeated column chromatography and their effect on aldose reductase (AR), obtained from rat lenses, was examined. Results showed that the ethyl acetate and n-butanol fractions of L. cuneata exhibited potential inhibitory effect against AR with IC50 values of 0.57 and 0.49 μg/mL, respectively. Phytochemical analysis of these two fractions resulted in the isolation of five flavonoids namely, acacetin (1), afzelin (2), astragalin (3), kaempferol (4) and scutellarein 7-O-glucoside (5). The AR inhibitory effect of compounds 1-5 was explored; compounds 2, 3 and 5 showed potential AR-inhibitory effects with IC50 values of 2.20, 1.91 and 12.87 μM, respectively. Quantitative analysis of afzelin (2) and astragalin (3) in L. cuneata by high performance liquid chromatography with ultraviolet detection revealed its content to be 0.722-11.828 and 2.054-7.006 mg/g, respectively. Overall, this study showed that L. cuneata is rich in flavonoids with promising AR-inhibitory activities, which can be utilized for the development of natural therapies for treating and managing diabetic complications.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/chromsci/bmaa127 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Phytochemical Profile, Antioxidant, Anti-Atopic, and Anti-Inflammatory Activities of Nakai at Different Growth Stages.
Pharmaceuticals (Basel)
July 2024
Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
Since atopic dermatitis is an inflammatory skin disease, natural remedies, such as Nakai (FG), with anti-inflammatory properties are possible promising therapeutic options. This study aimed to investigate the therapeutic potential of FG extracts at different growth stages. Seven compounds were isolated from the FG leaf extracts using open-column chromatography, and they were analyzed using HPLC.
View Article and Find Full Text PDFMolecular Docking Analysis of Selected Urtica dioica Constituents As Human Carbonic Anhydrase II (hCA-II), Human 11 Beta-Hydroxysteroid Dehydrogenases Type 1 (h11beta-HSD1), and Human Dual Specificity Phosphatase (hCDC25B) Inhibitory Agents.
Cureus
February 2024
Biochemistry, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences (Deemed to be University), Chennai, IND.
Background (Stinging nettle)has been reported to exhibit various pharmacological activities. In the present study, we aimed to evaluate 24 selected constituents of as potent inhibitory agents of human carbonic anhydrase II (hCA-II), human 11 beta-hydroxysteroid dehydrogenases type 1 (h11beta-HSD1), and human dual specificity phosphatase (hCDC25B) using method. Methodology The 24 selected constituents of (Stinging nettle) were studied on the docking behavior of hCA-II, h11beta-HSD1, and hCDC25B by using the Webina docking method.
View Article and Find Full Text PDFA study for quality evaluation of Lysimachiae herba from different origins based on fingerprint-activity relationship modeling and multi-component content determination.
J Ethnopharmacol
May 2024
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China; Jiangsu Province Engineering Research Center of Classical Prescription, Nanjing 210023, China.
Article Synopsis
- Lysimachiae Herba (LH), a traditional Chinese medicine known for its bitter and slightly cold properties, is effective in treating conditions like jaundice, gallstones, and liver issues, but quality evaluation methods for its different origins remain insufficient.
- The study aimed to create a model linking the chemical fingerprint of LH to its protective effects against cholestatic liver injury, using samples from Sichuan and Guizhou, and employed advanced analysis techniques to assess their quality.
- Results showed that 15 common peaks were identified in the chemical fingerprint of LH samples, indicating a high similarity among them and contributing to the understanding of the pharmacological effectiveness of LH.
Dataset on the compounds from the leaves of Vietnamese and their anti-inflammatory activity.
Data Brief
December 2023
Faculty of Basic Sciences, Can Tho University of Medicine and Pharmacy, 179 Nguyen Van Cu, An Khanh Ward, Ninh Kieu District, Can Tho 94000, Vietnam.
has a history of traditional applications including treating dyspepsia, apoplexy, headaches, abdominal pain, abdominal distension, and leg edema [1]. It is also employed for alleviating allergies, inflammation, pain relief, promoting blood circulation, addressing costal chondritis, and sinusitis [2]. Research into the chemical composition of has revealed the presence of lignans, flavonoids, lactones, and essential oils [1,[3], [4], [5].
View Article and Find Full Text PDFEthnopharmacological impact of Melaleuca rugulosa (Link) Craven leaves extract on liver inflammation.
J Ethnopharmacol
June 2022
Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Ein Helwan, Cairo, 11795, Egypt.
Ethnopharmacological Relevance: Melaleuca species have been used by many ethnic communities for the management and treatment of several ailments as hemorrhoids, cough, skin infections, rheumatism, sore throat, pain, inflammation, and digestive system malfunctions. However, the detailed mechanistic pharmacological effect of Melaleuca rugulosa (Link) Craven leaves in the management of liver inflammation has not been yet addressed.
Aim Of The Study: The present study aimed to evaluate the anti-inflammatory, antioxidant, and antiapoptotic capacities of the aqueous methanol extract of M.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!