In vitro Interactions between and K12 on a Titanium Cylindrical Surface.

Peri-implantitis is a steadily rising disease and is caused by oral bacterial pathogens able to form biofilm on implant surfaces and peri-implant tissues, making antibiotics treatment less effective. The use of commercial probiotics against oral pathogens could serve as an alternative to prevent biofilm formation. is one of the early colonizers of biofilm formation in dental implants. The aim of this study was to model the interaction between and strain K12, a probiotic bacterium producing bacteriocins. was co-cultured with K12 in an in vitro model simulating the biofilm formation in a dental implant composed by a titanium cylinder system. Biofilm formation rate was assessed by Real-Time PCR quantification of bacterial count and expression levels of gene, used in response to cell density in the biofilm. Biofilm formation, bacteriocin production, expression patterns were found to be already expressed within the first 12 h. More importantly, K12 was able to counter the biofilm formation in a titanium cylinder under the tested condition. In conclusion, our dental implant model may be useful for exploring probiotic-pathogen interaction to find an alternative to antibiotics for peri-implantitis treatment.