AI Article Synopsis
- The study evaluates the safety of eight nucleic acid dyes (EB, GelGreen, GoldView, GeneGreen, SYBRGreen I, GelRed, SYBRSafe, and SYBRGold) using Salmonella strains to determine their mutagenicity.
- Results indicate that Ethidium Bromide (EB) is significantly mutagenic, while SYBRGold and its metabolites show no mutagenicity.
- Other dyes exhibit varying levels of mutagenicity, but most are safe at working concentrations; however, SYBRSafe and SYBRGreen I are highly toxic and require careful handling.
Article Abstract
Objective: To study the safety of eight nucleic acid dyes: EB, GelGreen, GoldView, GeneGreen, SYBRGreen I, GelRed, SYBRSafe and SYBRGold.
Methods: Salmonell atyphimurium TA97, TA98, TA100 and TA102 involved in the minimum inhibitory concentration(MIC) of the 8 nucleic acid dyes by the Broth dilution, which follows Clinical and Laboratory Standards Institute. The experiment tests the reverse mutation of bacteria with the 8 nucleic acid dyes, which follows GB 15193. 4-2014.
Results: EB and its metabolites show significant mutagenicity on TA97, TA98, TA100 and TA102. SYBRGold and its metabolites do not show mutagenicity on TA97, TA98, TA100 and TA102. The other six dyes show varying mutagenicity on TA97, TA98 and TA102, meanwhile show no mutagenicity on TA100.
Conclusion: This research shows that, except EB, the other dyes show no mutagenicity under working concentration, but show varying mutagenicity under high concentration. SYBRSafe and SYBRGreenI are highly toxic, and the operators must have high precautions when making diluted solution of them.
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| http://dx.doi.org/10.19813/j.cnki.weishengyanjiu.2020.06.011 | DOI Listing |
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