AI Article Synopsis
- Zearalenone (ZEN) exposure negatively affects oocyte quality by impairing preimplantation developmental potential, but the specific mechanisms and lowest effective levels are still unclear.
- The study tested the effects of varying doses of invivo ZEN on mouse oocytes and found that ZEN increases oxidative stress and disrupts chromatin configuration, spindle assembly, and gene transcription related to oocyte competence.
- Results indicated that ZEN reduces the competency of mouse oocytes in a dose-dependent manner through oxidative stress and changes in chromatin and gene expression.
Article Abstract
Although invivo and invitro zearalenone (ZEN) exposure impaired oocyte quality, the mechanisms by which ZEN damages oocytes and the lowest observed effect level remain unclear. Furthermore, although it is known that premature chromatin condensation may occur in oocytes under proapoptotic conditions, whether ZEN exposure compromises oocyte competence by impairing gene transcription by causing premature chromatin condensation remains to be investigated. This study tested the toxic concentrations of invivo ZEN exposure that impair oocyte preimplantation developmental potential (PIDP) and the hypothesis that ZEN exposure compromises oocyte competence by increasing oxidative stress and changing chromatin configuration and the transcription of related genes. We found that invivo treatment of mice (Kunming strain, 8 weeks after birth) with 0.5-1mg kg-1 ZEN daily for 5 days, impaired the PIDP of mouse oocytes, increased oxidative stress, disturbed spindle assembly and chromosome segregation, caused premature chromatin condensation, impaired global gene transcription and disturbed the expression of genes related to oocyte competence, spindle assembly, redox potential and apoptosis. In conclusion, ZEN dose-dependently compromised the competence of mouse oocytes by causing oxidative stress and impairing chromatin configuration and gene transcription.
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| http://dx.doi.org/10.1071/RD20273 | DOI Listing |
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