The objective of this study is to analyze the association between combinations of adherence to movement behaviour recommendations and fundamental movement skills (FMS) in preschoolers. This is a cross-sectional study. Participants of the study were 212 preschool children (M = 3.97 years old; 51.4% male), who provided objectively assessed physical activity (PA) data (Actigraph wGT3X), and completed FMS assessments (TGMD-2). Sleep time and screen time were parent-reported through face-to-face interview. Associations between the combination of two or three movement behaviours and FMS were analyzed using structural equation modeling (Mplus; 8.0; p < 0.05). Positive and significant associations were found between adherence to screen + sleep recommendations and locomotor skills (β = 0.23; p = 0.027); and between adherence to PA + screen + sleep recommendations with object control skills (β = 0.28; p = 0.014). Negative and significant associations were found between screen + sleep with object control skills (β = - 0.28; p = 0.007). The adherence to the 24-h movement behaviour recommendations explained locomotor and object control skills variability by 5% and 7%, respectively.Conclusion: The adherence to the combined movement behaviour recommendations may be a more important influence on FMS in preschoolers compared to any single movement behaviour in isolation. What is Known: • The association between the isolated adherence to movement behaviour (physical activity, screen time and sleep time) recommendations and fundamental movement skills (FMS) in preschoolers, and the role of the 24-h movement behaviours on FMS, has been previously reported. What is New: • This study adds important information to the current literature, when highlighting that the combined adherence to physical activity, screen time and sleep time recommendations is positively associated with object control skills (such as throwing and kicking), and the combination of screen time and sleep is positively associated with locomotor skills (such as running and hopping).

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