Evaluation of commercial virtually imaged phase array and Fabry-Pérot based Brillouin spectrometers for applications to biology.

Biomed Opt Express

Institut Lumière Matière, UMR5306, Université Lyon 1-CNRS, Université de Lyon, 69622 Villeurbanne, France.

Published: December 2020

Measuring the complex mechanical properties of biological objects has become a necessity to answer key questions in mechanobiology and to propose innovative clinical and therapeutic strategies. In this context, Brillouin light scattering (BLS) has recently come into vogue, offering quantitative imaging of the mechanical properties without labels and with a micrometer resolution. In biological samples, the magnitude of the spectral changes are typically of a few tens of MHz, and the ability of modern spectrometers to monitor such subtle changes needs to be evaluated. Moreover, the multiplicity of variations in optical arrangements, specific to each lab, requires to set a standard for the assessment of the characteristics of BLS systems. In this paper we propose a protocol to evaluate the precision and accuracy of two commercial spectrometers that is reproducible across labs. For a meaningful comparison, we coupled the spectrometers to the same microscope and to the same laser. We first evaluated the optimum acquisition time and laser power. We evaluated the precision using pure water samples. We determined the accuracy by probing water solutions with increasing concentration of salt and comparing it with theory. Following these quantifications, we applied the VIPA-based spectrometer to tumor spheroids engineered from different cell lines that possess different metastatic potentials and resistance to therapies. On these models, we detected significant changes in the linewidth suggesting that BLS measurements of the viscosity could be used as a read-out to distinguish different levels of drug resistance.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7747923PMC
http://dx.doi.org/10.1364/BOE.401087DOI Listing

Publication Analysis

Top Keywords

mechanical properties
8
evaluation commercial
4
commercial virtually
4
virtually imaged
4
imaged phase
4
phase array
4
array fabry-pérot
4
fabry-pérot based
4
based brillouin
4
spectrometers
4

Similar Publications

Chronic pain and restricted mobility, hallmark features of rheumatic diseases, substantially affect patients' quality of life, often resulting in physical disability and emotional distress. Given the long-term nature of these conditions, there is a growing interest in complementary therapeutic approaches, emphasizing the need to explore non-pharmacological treatments. Hydrotherapy, balneotherapy, and mud therapy have emerged as effective interventions to alleviate pain, reduce inflammation, improve joint mobility, and enhance overall physical and mental well-being.

View Article and Find Full Text PDF

The CRTS (China Railway Track System) II slab ballastless track is widely utilized in high-speed railway construction owing to its excellent structural integrity. However, its interfacial performance deteriorates under high-temperature conditions, leading to significant damage in structural details. Furthermore, the evolution of its performance under these conditions has not been comprehensively studied.

View Article and Find Full Text PDF

It has long been speculated that the mechanical properties of the human oocyte can be an indicator for oocyte viability. Recent studies have demonstrated that embryo implantation rates, following Intra-Cytoplasmic Sperm Injection (ICSI) procedures, may be increased if the shear modulus value of the oocyte Zona Pellucida (ZP) is taken into consideration during embryo transfer. The shear modulus was determined by an iterative oocyte specific finite element (FE) analysis based on the clinical ICSI data.

View Article and Find Full Text PDF

Lubricants are pivotal in mitigating friction and wear between surfaces, ensuring seamless movement of solid objects. However, the predominant use of petroleum-based lubricants in the automotive and industrial ssectors raises substantial concerns for future energy security. The exploration of vegetable oils as an alternative lubricant in the automotive industry was motivated by the depletion of fossil fuels and escalating environmental concerns.

View Article and Find Full Text PDF

Today, there are environmental problems all over the world due to the emission of greenhouse gasses caused by the combustion of diesel fuel. The excessive consumption and drastic reduction of fossil fuels have prompted the leaders of various countries, including Iran, to put the use of alternative and clean energy sources on the agenda. In recent years, the use of biofuels and the addition of nanoparticles to diesel fuel have reduced pollutant emissions, improved the environment, and enhanced the physicochemical properties of the fuel.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!