In vivo haploid induction in high frequency followed by efficient identification of haploids are important components of deriving completely homozygous doubled haploid (DH) lines in maize. Several genetic marker systems were proposed and/or used for identification of in vivo maternal haploids in maize, such as (Navajo), high oil, red root and transgenic markers. In this study, we propose a new method of haploid/diploid identification based on natural differences in seedling traits of haploids and diploids, which can be used in any induction cross independently of the genetic marker systems. Using confirmed haploids and diploids from five different populations, the study established that haploid and diploid seedlings exhibit significant differences for seedling traits, particularly radicle length (RL), coleoptile length (CL), and number of lateral seminal roots (NLSR). In six populations that exhibited complete inhibition of the commonly used (Navajo) marker, we could effectively differentiate haploids from diploids by visual inspection of the seedling traits. In the haploid seed fraction identified based on marker in ten populations, false positives were reduced several-fold by early identification of haploids at seedling stage using the seedling traits. We propose that seedling traits may be integrated at the haploid identification stage, especially in populations that are not amenable to use of genetic markers, and for improving the efficiency of DH line production by reducing the false positives.
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http://dx.doi.org/10.1007/s10681-017-1968-3 | DOI Listing |
BMC Genomics
January 2025
Crop Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, China.
Leaf is the main photosynthetic organ at the seedling stage of rapeseed and leaf size is a crucial agronomic trait affecting rapeseed yield. Understanding the genetic mechanisms underlying leaf size is therefore important for rapeseed breeding. In this study, QTL mapping for three traits related to leaf size, i.
View Article and Find Full Text PDFJ Environ Manage
January 2025
School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan, 410083, China. Electronic address:
Boron (B) is essential for plant growth and helps mitigate metal toxicity in various crop plants. However, the potential role and underlying mechanisms of B in alleviating antimony (Sb) toxicity in rice remain unexplored. In this study, we investigated the effects of H₃BO₃ supplementation (30, 50, and 75 μM) on morphological growth, physiological and biochemical traits, Sb content, and the subcellular distribution of Sb in rice plants under 100 μM Sb stress during the seedling stage in a hydroponic system.
View Article and Find Full Text PDFPhotosynthetica
January 2025
College of Life Science, Northwest Normal University, 730070 Lanzhou, China.
This study aimed to explore the mechanism by which Zn retards Fe toxicity by analyzing the morphological, photosynthetic, and chloroplast physiological parameters of wheat seedlings treated with either single or combined Zn and Fe. Different behavior of the seedlings was observed under untreated and treated conditions. The most discriminating quantitative traits were associated with leaf area, biomass dry mass and fresh mass, net photosynthetic rate, intercellular CO concentration, stomatal conductance, transpiration rate of seedlings, Hill reaction, Mg-ATPase and Ca-ATPase activities, malondialdehyde and O contents, and glutathione reductase, ascorbate peroxidase, peroxidase, and superoxide dismutase activities and their gene expression in the seedling chloroplast.
View Article and Find Full Text PDFPlanta
January 2025
Laboratory of Environmental Microbiology and Biotechnology, Universidade Vila Velha (UVV), Vila Velha, ES, Brazil.
Both, Serendipita indica and AMF, show promise as sustainable biofertilizers for reforestation, improving nutrient uptake and stress tolerance, despite contrasting effects on photosynthetic capacity and biomass allocation. Reclaiming degraded areas is essential for biodiversity conservation and enhancing ecosystem services enhancement, especially when using native species. This study investigated Schinus terebinthifolius Raddi, a native Brazilian species, and its compatibility with plant growth-promoting microorganisms (PGPM), including an endophytic fungus (Serendipita indica) and a consortium of arbuscular mycorrhizal fungi (AMF), to identify effective strategies for reforestation in nutrient-poor environments.
View Article and Find Full Text PDFEnviron Microbiome
January 2025
School of Natural Sciences, Macquarie University, Sydney, NSW, Australia.
Background: Seed banks are a vital resource for preserving plant species diversity globally. However, seedling establishment and survival rates from banked seeds can be poor. Despite a growing appreciation for the role of seed-associated microbiota in supporting seed quality and plant health, our understanding of the effects of conventional seed banking processes on seed microbiomes remains limited.
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