AI Article Synopsis

  • In vivo haploid induction and identification are crucial for creating fully homozygous doubled haploid (DH) lines in maize, which is essential for maize breeding.
  • The study introduces a new method to distinguish haploids from diploids based on observable differences in seedling traits like radicle length, coleoptile length, and number of lateral roots, rather than relying solely on genetic markers.
  • By applying this method in six populations where traditional markers were ineffective, researchers significantly reduced the likelihood of false positives when identifying haploids, improving the efficiency of DH line production.

Article Abstract

In vivo haploid induction in high frequency followed by efficient identification of haploids are important components of deriving completely homozygous doubled haploid (DH) lines in maize. Several genetic marker systems were proposed and/or used for identification of in vivo maternal haploids in maize, such as (Navajo), high oil, red root and transgenic markers. In this study, we propose a new method of haploid/diploid identification based on natural differences in seedling traits of haploids and diploids, which can be used in any induction cross independently of the genetic marker systems. Using confirmed haploids and diploids from five different populations, the study established that haploid and diploid seedlings exhibit significant differences for seedling traits, particularly radicle length (RL), coleoptile length (CL), and number of lateral seminal roots (NLSR). In six populations that exhibited complete inhibition of the commonly used (Navajo) marker, we could effectively differentiate haploids from diploids by visual inspection of the seedling traits. In the haploid seed fraction identified based on marker in ten populations, false positives were reduced several-fold by early identification of haploids at seedling stage using the seedling traits. We propose that seedling traits may be integrated at the haploid identification stage, especially in populations that are not amenable to use of genetic markers, and for improving the efficiency of DH line production by reducing the false positives.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734196PMC
http://dx.doi.org/10.1007/s10681-017-1968-3DOI Listing

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