Background: The aim of this study was the investigation of the osteogenic potential of human osteoblasts of advanced donor age in 2D and 3D culture.
Methods: Osteoblasts were induced to osteogenic differentiation and cultivated, using the same polystyrene material in 2D and 3D culture for 2 weeks. Samples were taken to evaluate alkaline phosphatase (ALP) activity, mineralization and gene expression.
Results: Osteoprotegerin (OPG) levels were significantly increased (8.2-fold) on day 7 in 3D compared to day 0 (p < 0.0001) and 11.6-fold higher in 3D than in 2D (p < 0.0001). Both culture systems showed reduced osteocalcin (OC) levels (2D 85% and 3D 50% of basic value). Collagen type 1 (Col1) expression was elevated in 3D on day 7 (1.4-fold; p = 0.009). Osteopontin (OP) expression showed 6.5-fold higher levels on day 7 (p = 0.002) in 3D than in 2D. Mineralization was significantly higher in 3D on day 14 (p = 0.0002).
Conclusion: Advanced donor age human primary osteoblasts reveal significantly higher gene expression levels of OPG, Col1 and OP in 3D than in monolayer. Therefore, it seems that a relatively high potential of bone formation in a natural 3D arrangement is presumably still present in osteoblasts of elderly people.
Trial Registration: 5217/11 on the 22nd of Dec. 2011.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7788858 | PMC |
| http://dx.doi.org/10.1186/s13018-020-02153-z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Opportunities to Digitally Enable Falls Prevention in Older Adults.
Gerontologist
January 2025
Health and Social Care Unit, School of Public Health and Preventive Medicine, Monash University, Melbourne, Australia.
Falls are a serious problem confronting older adults. Evidence demonstrates that multifactorial interventions that target multiple risk factors can reduce falls. However, resource and access constraints impact intervention uptake and sustainability.
View Article and Find Full Text PDFIncorporating Multimodal Directional Interpersonal Synchrony into Empathetic Response Generation.
Sensors (Basel)
January 2025
Faculty of Engineering, University of Toyama, Toyama 930-8555, Japan.
This study investigates how interpersonal (speaker-partner) synchrony contributes to empathetic response generation in communication scenarios. To perform this investigation, we propose a model that incorporates multimodal directional (positive and negative) interpersonal synchrony, operationalized using the cosine similarity measure, into empathetic response generation. We evaluate how incorporating specific synchrony affects the generated responses at the language and empathy levels.
View Article and Find Full Text PDFA Review on Face Mask Recognition.
Sensors (Basel)
January 2025
School of Information and Communications Engineering, Xi'an Jiaotong University, Xi'an 710049, China.
This review offers a comprehensive and in-depth analysis of face mask detection and recognition technologies, emphasizing their critical role in both public health and technological advancements. Existing detection methods are systematically categorized into three primary classes: feaRture-extraction-and-classification-based approaches, object-detection-models-based methods and multi-sensor-fusion-based methods. Through a detailed comparison, their respective workflows, strengths, limitations, and applicability across different contexts are examined.
View Article and Find Full Text PDFRelationship Between Depression and Decreased Activity Level and Cognitive Impairment in Patients with Diabetes Mellitus Type 2 and/or Atrial Fibrillation.
J Clin Med
January 2025
Emergency City Hospital Timisoara, Gheorghe Dima Street, Nr. 5, 300254 Timisoara, Romania.
The interdependence between type 2 diabetes mellitus (DM-2), atrial fibrillation (AF), and cognitive decline (CD)/dementia is a debated topic. In this study, we highlighted the influence of DM-2 and FA individually and in association on the severity of CD/dementia. This study comprises 248 patients with very high cardiovascular risk (VHCVR) according to Systematic Coronary Risk Evaluation (SCORE2), of whom 184 had DM-2 and/or AF, and 64 were age-matched controls (without DM-2/AF), admitted to the Municipal Hospital Timisoara.
View Article and Find Full Text PDFComparison of In Situ and Postsynthetic Formation of MOF-Carbon Composites as Electrocatalysts for the Alkaline Oxygen Evolution Reaction (OER).
Molecules
January 2025
Institut für Anorganische Chemie und Strukturchemie, Heinrich-Heine-Universität Düsseldorf, 40225 Düsseldorf, Germany.
Mixed-metal nickel-iron, NiFe materials draw attention as affordable earth-abundant electrocatalysts for the oxygen evolution reaction (OER). Here, nickel and mixed-metal nickel-iron metal-organic framework (MOF) composites with the carbon materials ketjenblack (KB) or carbon nanotubes (CNT) were synthesized in situ in a one-pot solvothermal reaction. As a direct comparison to these in situ synthesized composites, the neat MOFs were postsynthetically mixed by grinding with KB or CNT, to generate physical mixture composites.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!