Methacrylated gelatin (Gel-MA) is a commonly used biomaterial in bioprinting applications. The Gel-MA synthesis procedure is inadequate and needs to be improved, particularly from the point of optimization and efficacy. We report a significantly faster (by 5 min) and effective method to controllably synthesize Gel-MA using microwave energy (Mw at 1000 W power) with ≥90% degree of methacrylation (DM) even with the use of a very low concentration of methacrylic anhydride (MA). Rheological and mechanical analyses indicated that Gel-MA synthesized by Mw-assisted methacrylation enabled the formation of hydrogels that are more elastic and stronger and have a lower degradation rate (∼27% at 35 days) than Gel-MA synthesized by the conventional method. The viscosity values of the Gel-MA bioink were in the range applicable for use in 3D bioprinters. Additionally, Mw-assisted methacrylated Gel-MA hydrogels that have mechanically superior properties significantly enhanced the viability, attachment, proliferation, alkaline phosphatase (ALP) activity, mineral deposition, and mRNA expression levels of osteogenic genes of MC3T3-E1 preosteoblastic cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acsbiomaterials.8b00778 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Magnetic Nanoactuator-Protein Fiber Coated Hydrogel Dressing for Well-Balanced Skin Wound Healing and Tissue Regeneration.
ACS Nano
January 2025
State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, School of Medicine, Tongji University, Shanghai 200092, P. R. China.
Despite significant progress in skin wound healing, it is still a challenge to construct multifunctional bioactive dressings based on a highly aligned protein fiber coated hydrogel matrix for antifibrosis skin wound regeneration that is indistinguishable to native skin. In this study, a "dual-wheel-driven" strategy is adopted to modify the surface of methacrylated gelatin (GelMA) hydrogel with highly aligned magnetic nanocomposites-protein fiber assemblies (MPF) consisting of photothermal responsive antibacteria superparamagnetic nanocomposites-fibrinogen (Fg) complexes as the building blocks. Whole-phase healing properties of the modified hydrogel dressing, GelMA-MPF (GMPF), stem from the integration of Fg protein with RGD peptide activity decorated on the surface of the antibacterial magnetic nanoactuator, facilitating facile and reproducible dressing preparation by self-assembly and involving biochemical, morphological, and biophysical cues.
View Article and Find Full Text PDFPhotothermal Effect and Biomineralization of Black Phosphorus Nanosheet-Composited Hydrogel Boosts Synergistic Treatment of Dentin Hypersensitivity.
Adv Sci (Weinh)
January 2025
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, 130022, P. R. China.
Dentin hypersensitivity (DH), marked by exposed dentinal tubules, presents as a sharp toothache triggered by stimuli and subsides when the stimuli are removed. To address the limitations of current commercial desensitizers in terms of acid resistance, friction resistance, and stability, a black phosphorus nanosheet-composited methacrylate gelatin hydrogel (GelMA/BP) is developed for DH treatment, leveraging the synergistic effects of photothermal therapy and biomineralization. Incorporating the BP nanosheet provided GelMA/BP with a stable photothermal response and the continuous release of phosphate anions, which blocked dentinal tubules by converting light energy into heat and initiating biomineralization.
View Article and Find Full Text PDFDouble network hydrogels encapsulating genetically modified dedifferentiated chondrocytes for auricular cartilage regeneration.
J Mater Chem B
January 2025
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
Microtia profoundly affects patients' appearance and psychological well-being. Tissue engineering ear cartilage scaffolds have emerged as the most promising solution for ear reconstruction. However, constructing tissue engineering ear cartilage scaffolds requires multiple passaging of chondrocytes, resulting in their dedifferentiation and loss of their special phenotypes and functions.
View Article and Find Full Text PDF4D bioprinting of transformable living constructs with sustained local growth factor presentation for advanced tissue engineering applications.
Colloids Surf B Biointerfaces
December 2024
Cardiovascular Center, The First Hospital of Jilin University, Changchun, Jilin 130021, China. Electronic address:
Traditional tissue engineering strategies focus on geometrically static tissue scaffolds, lacking the dynamic capability found in native tissues. The emerging field of 4D bioprinting offers a promising method to address this challenge. However, the requirement for consistent exogenous supplementation of growth factors (GFs) during tissue maturation poses a significant obstacle for in vivo application of 4D bioprinted constructs.
View Article and Find Full Text PDFCo-culturing neural and bone mesenchymal stem cells in photosensitive hydrogel enhances spinal cord injury repair.
Front Bioeng Biotechnol
December 2024
Department of Orthopedics, The First Affiliated Hospital of Soochow University, Soochow University, Suzhou, Jiangsu, China.
In mammalian species, neural tissues cannot regenerate following severe spinal cord injury (SCI), for which stem cell transplantation is a promising treatment. Neural stem cells (NSCs) have the potential to repair SCI; however, in unfavourable microenvironments, transplanted NSCs mainly differentiate into astrocytes rather than neurons. In contrast, bone mesenchymal stem cells (BMSCs) promote the differentiation of NSCs into neurons and regulate inflammatory responses.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!