Structural determination of molecular complexes by cryo-EM requires large, often complex processing of the image data that are initially obtained. Here, TEMPy2, an update of the TEMPy package to process, optimize and assess cryo-EM maps and the structures fitted to them, is described. New optimization routines, comprehensive automated checks and workflows to perform these tasks are described.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7787107PMC
http://dx.doi.org/10.1107/S2059798320014928DOI Listing

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