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In vivo confocal microscopy findings about ocular surface recovery in patients following pterygium excision combined with conjunctival autograft.
Int Ophthalmol
December 2024
Department of Ophthalmology, Jiangnan University Medical Center, Wuxi No.2 People's Hospital, Affiliated Wuxi Clinical College of Nantong University, Wuxi, 214000, People's Republic of China.
Purpose: To study cellular and neural changes around the donor and recipient areas after pterygium excision combined with autologous conjunctival transplantation using corneal confocal microscopy, we evaluated the postoperative impact of this surgical procedure.
Methods: Patients with primary pterygium located on the nasal side who visited the Department of Ophthalmology of Wuxi Second People's Hospital between April and August 2023 were enrolled. Age-matched healthy eyes were included as the control group.
Outcomes of staged conjunctival flap and corneal transplantation for infectious keratitis resistant to medical treatment.
Br J Ophthalmol
December 2024
Department of Translational Medicine and for Romagna, University of Ferrara, Ferrara, Italy.
Background/aims: To explore the outcomes of conjunctival flap (CF) followed by keratoplasty in patients with severe infectious keratitis (IK) unresponsive to medical treatment.
Methods: This is a retrospective monocentric study including 29 eyes of 29 patients with IK resistant to antibiotic treatment associated with stromal ulcer deepening to ≥50% of corneal thickness. All eyes underwent Gundersen's CF procedure and penetrating keratoplasty (PK), deep anterior lamellar keratoplasty (DALK) or mushroom keratoplasty (MK), at least 4 months thereafter.
Enrichment protocols for human conjunctival extracellular vesicles and their characterization.
Sci Rep
November 2024
Ocular Surface Group, Instituto Universitario de Oftalmobiología Aplicada (IOBA), Universidad de Valladolid, Valladolid, Spain.
The understanding of the role played by extracellular vesicles (EVs) in different tissues has improved significantly in the last years, but remains limited concerning the conjunctiva, a complex eye tissue whose role is pivotal for corneal protection. Here, we conducted a comparative study to isolate and characterize EVs from human conjunctival epithelial (IM-HConEpiC) and human conjunctival mesenchymal stromal cell (Conj-MSCs) secretomes using different isolation methods: differential ultracentrifugation (UC), and a combination of ultrafiltration (UF) with precipitation or size exclusion chromatography (SEC). EVs were characterized by total protein content, size, morphology, and expression of protein markers.
View Article and Find Full Text PDFAdipose-derived mesenchymal stromal cells: A study on safety and efficacy in ocular inflammation.
Ocul Surf
October 2024
Department of Clinical Regenerative Medicine, Fujita Health University, Japan. Electronic address:
Purpose: This study explores the application of adipose-derived mesenchymal stromal cells (adMSCs) as a therapy for ocular inflammatory diseases utilizing a chronic GVHD model.
Methods: Human adMSCs were administered via subconjunctival injection into mice with chronic ocular GVHD. Clinical scores and changes in T cell populations were analyzed.
Ocular involvement of oak processionary caterpillar hairs: Clinical outcome up to one year.
Graefes Arch Clin Exp Ophthalmol
November 2024
Department of Ophthalmology, University Medical Center Muenster, Muenster, Germany.
Purpose: To evaluate a consecutive series of patients that presented with ocular findings after contact with the oak processionary caterpillar (OPC) during an epidemic reproduction of the OPC in Germany in 2019 and to assess the 1-year outcome of those eyes with persisting OPC hairs in the cornea.
Methods: Retrospective analysis of 11 eyes (11 patients) that presented in June/July 2019 with acute ocular symptoms after outdoor activity or caterpillar nest removal. Evaluation of patients charts and slit-lamp images up to one year.
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