The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its receptor binding domain in two conformations, the receptor-accessible 'up' or receptor-inaccessible 'down' states. Here we report that the commonly used stabilized S ectodomain construct '2P' is sensitive to cold temperatures, and this cold sensitivity is abrogated in a 'down' state-stabilized ectodomain. Our findings will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7878407 | PMC |
| http://dx.doi.org/10.1038/s41594-020-00547-5 | DOI Listing |
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