Meiosis creates genetic diversity by recombination and segregation of chromosomes. The synaptonemal complex assembles during meiotic prophase I and assists faithful exchanges between homologous chromosomes, but how its assembly/disassembly is regulated remains to be understood. Here, we report how two major posttranslational modifications, phosphorylation and ubiquitination, cooperate to promote synaptonemal complex assembly. We found that the ubiquitin ligase complex SCF is important for assembly and maintenance of the synaptonemal complex in Drosophila female meiosis. This function of SCF is mediated by two substrate-recognizing F-box proteins, Slmb/βTrcp and Fbxo42. SCF-Fbxo42 down-regulates the phosphatase subunit PP2A-B56, which is important for synaptonemal complex assembly and maintenance.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7780726 | PMC |
| http://dx.doi.org/10.1083/jcb.202009167 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The centromere effect (CE) is a meiotic phenomenon that ensures meiotic crossover suppression in pericentromeric regions. Despite being a critical safeguard against nondisjunction, the mechanisms behind the CE remain unknown. Previous studies have shown that various regions of the pericentromere, encompassing proximal euchromatin, beta and alpha heterochromatin, undergo varying levels of crossover suppression, raising the question of whether distinct mechanisms establish the CE in these different regions.
View Article and Find Full Text PDFThe ATPase activity of yeast chromosome axis protein Hop1 affects the frequency of meiotic crossovers.
Nucleic Acids Res
December 2024
Department of Biochemistry, Indian Institute of Science, CV Raman Road, Bengaluru 560012, India.
Saccharomyces cerevisiae meiosis-specific Hop1, a structural constituent of the synaptonemal complex, also facilitates the formation of programmed DNA double-strand breaks and the pairing of homologous chromosomes. Here, we reveal a serendipitous discovery that Hop1 possesses robust DNA-independent ATPase activity, although it lacks recognizable sequence motifs required for ATP binding and hydrolysis. By leveraging molecular docking combined with molecular dynamics simulations and biochemical assays, we identified an ensemble of five amino acid residues in Hop1 that could potentially participate in ATP-binding and hydrolysis.
View Article and Find Full Text PDFIdentification of the Polo-like kinase substrate required for homologous synapsis.
J Cell Biol
March 2025
Department of Biology, Johns Hopkins University, Baltimore, MD, USA.
The synaptonemal complex (SC) is a zipper-like protein structure that aligns homologous chromosome pairs and regulates recombination during meiosis. Despite its conserved appearance and function, how synapsis occurs between chromosome axes remains elusive. Here, we demonstrate that Polo-like kinases (PLKs) phosphorylate a single conserved residue in the disordered C-terminal tails of two paralogous SC subunits, SYP-5 and SYP-6, to establish an electrostatic interface between the SC central region and chromosome axes in C.
View Article and Find Full Text PDFSpermatogenesis and sperm ultrastructure of the squid, Todarodes pacificus (Cephalopoda: Ommastrephidae).
Micron
February 2025
Department of Aqualife Medicine, Chonnam National University, Yeosu 59626, Republic of Korea. Electronic address:
Article Synopsis
- - The study focuses on spermatogenesis in Todarodes pacificus, identifying four stages: spermatogonium, spermatocyte, spermatid, and mature sperm.
- - Key characteristics of each stage are noted, including changes in karyoplasm and the development of structures such as mitochondria and proacrosomal vesicles during spermiogenesis.
- - Mature sperm are detailed with a specific nucleus size, irregular karyoplasm shape, and distinct acrosome and flagellum structures essential for function.
The Germline-Restricted Chromosome of Male Zebra Finches in Meiotic Prophase I: A Proteinaceous Scaffold and Chromatin Modifications.
Animals (Basel)
November 2024
Vavilov Institute of General Genetics, Russian Academy of Sciences, 119991 Moscow, Russia.
Among eukaryotes, there are many examples of partial genome elimination during ontogenesis. A striking example of this phenomenon is the loss of entire avian chromosomes during meiosis, called a germline-restricted chromosome (GRC). The GRC is absent in somatic tissues but present in germ cells.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!