AI Article Synopsis
- Genome mining revealed that bacterial strains sp. SH-C52 and DSM 11579 share a similar gene cluster for a nonribosomal peptide synthetase (NRPS), linked to a novel compound.
- Mutagenesis of the NRPS led to the identification and isolation of a new linear octalipopeptide named thanafactin A, with its structure confirmed using advanced techniques like two-dimensional NMR.
- Thanafactin A showed weak protease inhibitory effects and influenced swarming motility differently depending on the bacterial strain.
Article Abstract
Genome mining of the bacterial strains sp. SH-C52 and DSM 11579 showed that both strains contained a highly similar gene cluster encoding an octamodular nonribosomal peptide synthetase (NRPS) system which was not associated with a known secondary metabolite. Insertional mutagenesis of an NRPS component followed by comparative profiling led to the discovery of the corresponding novel linear octalipopeptide thanafactin A, which was subsequently isolated and its structure determined by two-dimensional NMR and further spectroscopic and chromatographic methods. In bioassays, thanafactin A exhibited weak protease inhibitory activity and was found to modulate swarming motility in a strain-specific manner.
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| http://dx.doi.org/10.1021/acs.jnatprod.0c01174 | DOI Listing |
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