AI Article Synopsis
- The study investigates the genetic and epigenetic interactions between neural stem/progenitor cells (NSPCs) and endothelial cells (ECs), focusing on identifying specific molecules and their associated drugs.
- Using datasets from the GEO database, the research employs differential expression analysis to assess how NSPCs and ECs influence each other at the molecular level, revealing shared genes and miRNAs linked to these interactions.
- The findings highlight three miRNAs and six genes as significant crosstalk molecules and suggest several small molecular drugs and environmental chemicals that may target these miRNAs, potentially influencing angiogenesis (blood vessel formation) and neurogenesis (nerve cell development).
Article Abstract
Aim: This study is aimed at identifying genetic and epigenetic crosstalk molecules and their target drugs involved in the interaction between neural stem/progenitor cells (NSPCs) and endothelial cells (ECs).
Materials And Methods: Datasets pertaining to reciprocal mRNA and noncoding RNA changes induced by the interaction between NSPCs and ECs were obtained from the GEO database. Differential expression analysis (DEA) was applied to identify NSPC-induced EC alterations by comparing the expression profiles between monoculture of ECs and ECs grown in EC/NSPC cocultures. DEA was also utilized to identify EC-induced NSPC alterations by comparing the expression profiles between monoculture of NSPCs and NSPCs grown in EC/NSPC cocultures. The DEGs and DEmiRNAs shared by NSPC-induced EC alterations and EC-induced NSPC alterations were then identified. Furthermore, miRNA crosstalk analysis and functional enrichment analysis were performed, and the relationship between DEmiRNAs and small molecular drug targets/environment chemical compounds was investigated.
Results: One dataset (GSE29759) was included and analyzed in this study. Six genes (i.e., MMP14, TIMP3, LOXL1, CCK, SMAD6, and HSPA2), three miRNAs (i.e., miR-210, miR-230a, and miR-23b), and three pathways (i.e., Akt, ERK1/2, and BMPs) were identified as crosstalk molecules. Six small molecular drugs (i.e., deptropine, fluphenazine, lycorine, quinostatin, resveratrol, and thiamazole) and seven environmental chemical compounds (i.e., folic acid, dexamethasone, choline, doxorubicin, thalidomide, bisphenol A, and titanium dioxide) were identified to be potential target drugs of the identified DEmiRNAs.
Conclusion: To conclude, three miRNAs (i.e., miR-210, miR-230a, and miR-23b) were identified to be crosstalks linking the interaction between ECs and NSPCs by implicating in both angiogenesis and neurogenesis. These crosstalk molecules might provide a basis for devising novel strategies for fabricating neurovascular models in stem cell tissue engineering.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7758130 | PMC |
| http://dx.doi.org/10.1155/2020/6630659 | DOI Listing |
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