Plant diseases have a significant impact on global food security and the world's agricultural economy. Their early detection and classification increase the chances of setting up effective control measures, which is why the search for automatic systems that allow this is of major interest to our society. Several recent studies have reported promising results in the classification of plant diseases from RGB images on the basis of Convolutional Neural Networks (CNN). These studies have been successfully experimented on a large number of crops and symptoms, and they have shown significant advantages in the support of human expertise. However, the CNN models still have limitations. In particular, CNN models do not necessarily focus on the visible parts affected by a plant disease to allow their classification, and they can sometimes take into account irrelevant backgrounds or healthy plant parts. In this paper, we therefore develop a new technique based on a Recurrent Neural Network (RNN) to automatically locate infected regions and extract relevant features for disease classification. We show experimentally that our RNN-based approach is more robust and has a greater ability to generalize to unseen infected crop species as well as to different plant disease domain images compared to classical CNN approaches. We also analyze the focus of attention as learned by our RNN and show that our approach is capable of accurately locating infectious diseases in plants. Our approach, which has been tested on a large number of plant species, should thus contribute to the development of more effective means of detecting and classifying crop pathogens in the near future.
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http://dx.doi.org/10.3389/fpls.2020.601250 | DOI Listing |
Plant Cell Rep
January 2025
MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, 510631, China.
The three SDEs of CLas were expressed in citrus leaves by AuNPs-PEI mediated transient expression system, and promoted the proliferation of CLas and inhibited citrus immunity. Huanglongbing (HLB) is the most severe bacterial disease of citrus caused by Candidatus Liberibacter asiaticus (CLas). CLas suppress host immune responses and promote infection by sec-dependent effectors (SDEs), thus insight into HLB pathogenesis is urgently needed to develop effective management strategies.
View Article and Find Full Text PDFBMC Plant Biol
January 2025
School of Engineering, Dali University, Dali, Yunnan Province, China.
The homeotic transformation of stamens into pistil-like structures (pistillody) causes cytoplasmic male sterility (CMS). This phenomenon is widely present in plants, and might be induced by intracellular communication (mitochondrial retrograde signaling), but its systemic regulating mechanism is still unclear. In this study, morphological observation showed that the stamens transformed into pistil-like structures, leading to flat and dehiscent pistils, and fruit set decrease in sua-CMS (MS K326, somatic fusion between Nicotiana.
View Article and Find Full Text PDFCommun Biol
January 2025
Institute of Phytopathology, Research Centre for BioSystems, Land Use and Nutrition, Justus Liebig University Giessen, Heinrich-Buff-Ring 26, 35392, Giessen, Germany.
In vertebrates and plants, dsRNA plays crucial roles as PAMP and as a mediator of RNAi. How higher fungi respond to dsRNA is not known. We demonstrate that Magnaporthe oryzae (Mo), a globally significant crop pathogen, internalizes dsRNA across a broad size range of 21 to about 3000 bp.
View Article and Find Full Text PDFSci Rep
January 2025
Department of Clinical Biochemistry, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
This study is designed to assess the effect of root extract of P. ginseng on kidney tissue injury attributed to cisplatin and its molecular mechanism involved in this process in the AKI rat model. Twenty-four male Wistar rats were randomly allocated into 4 experimental groups including: the control group, the cisplatin group, the extract 100 mg/kg group, and the extract 200 mg/kg group.
View Article and Find Full Text PDFJ Proteomics
January 2025
Embrapa Recursos Genéticos e Biotecnologia, PBI, Av. W/5 Norte Final CEP 70770917, Brazil. Electronic address:
The label-free shotgun proteomics analysis carried out in this study aimed to understand the molecular mechanisms that contribute towards tomato susceptibility to Xanthomonas euvesicatoria pv. perforans (Xep). To achieve this, comparative proteomics was performed on susceptible inoculated plants with the bacterium and the control group (saline solution) at 24 and 48 h after inoculation (hai).
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