AI Article Synopsis
- Capsular polysaccharides (CPSs) are key factors in bacterial pathogenicity and are used in pneumococcal vaccines like Pneumovax and Prevnar, greatly reducing diseases in developed nations.
- Traditional vaccine production is expensive and not scalable for developing countries, prompting the need for a more affordable solution.
- This study proposes a new low-cost live vaccine using a recombinant attenuated system, successfully eliciting strong immune responses in mice and showing effective protection against pneumococcal infections.
Article Abstract
capsular polysaccharides (CPSs) are major determinants of bacterial pathogenicity. CPSs of different serotypes form the main components of the pneumococcal vaccines Pneumovax, Prevnar7, and Prevnar13, which substantially reduced the disease burden in developed countries. However, the laborious production processes of traditional polysaccharide-based vaccines have raised the cost of the vaccines and limited their impact in developing countries. The aim of this study is to develop a kind of low-cost live vaccine based on using the recombinant attenuated vaccine (RASV) system to protect against pneumococcal infections. We cloned genes for seven different serotypes of CPSs to be expressed by the RASV strain. Oral immunization of mice with the RASV-CPS strains elicited robust Th1 biased adaptive immune responses. All the CPS-specific antisera mediated opsonophagocytic killing of the corresponding serotype of in vitro. The RASV-CPS2 and RASV-CPS3 strains provided efficient protection of mice against challenge infections with either strain D39 or WU2. Synthesis and delivery of CPSs using the RASV strains provide an innovative strategy for low-cost pneumococcal vaccine development, production, and use.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7812815 | PMC |
| http://dx.doi.org/10.1073/pnas.2013350118 | DOI Listing |
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