Natural products from and their antimycobacterial and anti-inflammatory activity.

Tuberculosis (TB) remains a worldwide public health threat because of the emergence of resistant strains and subsequent inappropriate response to current therapy. We have been studying the plants' antimycobacterial and anti-inflammatory potential. Dichloromethane fraction (DCM) from Cham. showed high activity against (Mtb) H37Rv. In this context, DCM fraction and isolated compounds were investigated against Mtb H37Rv and M299 (MDR strain) and for their immunomodulatory and cytotoxicity actions. Orientin showed the best antimycobacterial effect against Mtb M299 MDR strain (MIC 15.4 ± 1.6 µg/mL), capacity of inhibiting NO production by macrophages (IC 6.5 ± 1.2 µg/mL) and no significant cytotoxicity. The antimycobacterial effect of orientin was also observed on Mtb H37Rv intracellular growth in RAW 264.7 macrophages (MIC 3.5 ± 1.1 and MIC 9.1 ± 1.0 µg/mL). This is the first report describing the antimycobacterial effect of orientin, in both extra- and intracellular growth.[Formula: see text].