Growth and offspring count are two commonly determined toxicological endpoints for chemical- or gene-induced developmental and reproductive effects in . Here, we present a protocol for a 96 h, medium-throughput assay, assessing both endpoints quantitatively within an automated framework using open-source software. The assay utilizes whole 96-well fluorescence images taken with a high-content screening system. Alternatively, conventional fluorescence images can also be utilized with only a few adjustments. For complete details on the use and execution of this protocol, please refer to Wittkowski et al. (2019).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757729 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2020.100224 | DOI Listing |
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