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Protocol to Induce Wounding and Measure Membrane Repair in Epidermis. | LitMetric

Protocol to Induce Wounding and Measure Membrane Repair in Epidermis.

STAR Protoc

Center for Stem Cell and Regenerative Medicine and Department of Cardiology of The Second Affiliated Hospital, Zhejiang University School of Medicine, 310058 Hangzhou, China.

Published: December 2020

AI Article Synopsis

  • Efficient membrane repair is crucial for the survival of both cells and animals.
  • The epidermal cell hpy7 is a key model for studying the mechanisms involved in membrane repair.
  • The protocol outlines methods for wounding the epidermis and techniques like trypan blue staining and confocal imaging to analyze membrane repair, referencing Meng et al. (2020) for detailed execution.

Article Abstract

Efficient membrane repair after injury is essential for cell and animal survival. epidermal cell hpy7 has emerged as a powerful genetic system to investigate the molecular mechanism of membrane repair . This protocol describes detailed approaches for how to perform wounding on the epidermis and how to examine membrane repair by trypan blue staining, confocal imaging, and data analysis. For details on the use and execution of this protocol, please refer to Meng et al. (2020).

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7757402PMC
http://dx.doi.org/10.1016/j.xpro.2020.100175DOI Listing

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