Dual RNA-sequencing is a powerful technique to assess both bacterial and host transcriptomes in an unbiased way. We developed a protocol to perform Dual RNA-seq on -derived macrophage populations infected with . Here, we provide a practical step-by-step guide to execute the protocol on Mtb-infected cells from a murine infection model. Our protocol can also be easily applied to perform Dual RNA-seq on -derived cells as well as different Mtb-infected host cell types. For complete details on the use and execution of this protocol, please refer to Pisu et al. (2020).
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756974 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2020.100123 | DOI Listing |
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