We developed a reproducible micropatterning method to manipulate and normalize cell shape and cell-cell separation on gold. We used methoxy polyethylene glycol thiol (PEG-SH) to create a self-assembled monolayer that can be oxidized at desired shapes through a photomask with deep UV light. The oxidized PEG can be coated with extracellular matrix proteins and seeded with cells adopting the pre-defined shape. The developed and analyzed surfaces can be used in a wide range of biophysical applications.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7756938 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2020.100106 | DOI Listing |
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