The Degree of Infection Affects the State of Macrophage Polarization through Crosstalk between ROS and HIF-1.

Methods: The expression of CD86, CD206, and HIF-1 in the gastric mucosa was evaluated through immunohistochemistry. RAW 264.7 cells were cocultured with at various multiplicities of infection (MOIs), and iNOS, CD86, Arg-1, CD206, and HIF-1 expression was detected by Western blot, PCR, and ELISA analyses. ROS expression was detected with the fluorescent probe DCFH-DA. Macrophages were also treated with the ROS inhibitor NAC or HIF-1 inhibitor YC-1.

Results: Immunohistochemical staining revealed that the macrophage polarization state was associated with the progression of gastric lesions and state of infection. The MOI of affected macrophage polarization, and enhanced the expression of ROS and HIF-1 in macrophages. A low MOI of promoted both the M1 and M2 phenotypes, while a high MOI suppressed the M2 phenotype. Furthermore, ROS inhibition attenuated HIF-1 expression and switched macrophage polarization from M1 to M2. However, HIF-1 inhibition suppressed ROS expression and inhibited both the M1 phenotype and the M2 phenotype. Inhibition of ROS or HIF-1 also suppressed the activation of the Akt/mTOR pathway, which was implicated in -induced macrophage polarization.

Conclusions: Macrophage polarization is associated with the progression of gastric lesions and state of infection. The MOI of influences the macrophage polarization state. Crosstalk between ROS and HIF-1 regulates -induced macrophage polarization via the Akt/mTOR pathway.