Lancet liver flukes of the genus Dicrocoelium (Trematoda: Digenea) are recognised parasites of domestic and wild herbivores. The aim of the present study was to confirm the species identity of Dicrocoeliid flukes collected from the Chitral valley in the Himalayan ranges of Pakistan. The morphology of 48 flukes belonging to eight host populations was examined; but overlapping traits prevented accurate species designation. Phylogenetic comparison of published D. dendriticum ribosomal cistron DNA, and cytochrome oxidase-1 (COX-1) mitochondrial DNA sequences with those from D. chinensis was performed to assess within and between species variation and re-affirm the use of species-specific single nucleotide polymorphism markers. PCR and sequencing of 34 corresponding fragments of ribosomal DNA and 14 corresponding fragments of mitochondrial DNA from the Chitral valley flukes, revealed 10 and 4 unique haplotypes, respectively. These confirmed for the first time the molecular species identity of Pakistani lancet liver flukes as D. dendriticum. This work provides a preliminary illustration of a phylogenetic approach that could be developed to study the ecology, biological diversity, and epidemiology of Dicrocoeliid lancet flukes when they are identified in new settings.
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http://dx.doi.org/10.1016/j.parint.2020.102276 | DOI Listing |
Environ Sci Pollut Res Int
January 2025
Department of Animal Sciences, Central University of Himachal Pradesh, Kangra, 176206, Himachal Pradesh, India.
Environmental factors play a fundamental role in shaping fish assemblage in aquatic ecosystems. The present study describes the fish assemblage structure on the spatial scale in Pong Reservoir, which lies in foothills of the Northwestern Himalaya within the river Beas basin. Through sophisticated enviro assessment techniques, using ArcGIS mapping, this study provides valuable insight into how physicochemical factors shape the fish assemblage in the reservoir.
View Article and Find Full Text PDFNat Prod Res
January 2025
Department of Botany, D.B.S. (PG) College, Dehradun, Uttarakhand, India.
The aim of the present study was to demonstrate the phytochemical characterisation, antioxidant and antimicrobial properties of methanol extracts of leaf parts of . The Phytochemical characterisation by GC-MS analysis revealed the presence of 30 phytoconstituents such as Methyl commate A (14.69%), Phytol (13.
View Article and Find Full Text PDFSci Rep
December 2024
Department of Historical Geology-Paleontology, Faculty of Geology and Geoenvironment, School of Earth Sciences, National and Kapodistrian University of Athens, 15784, Panepistimiopolis, Zografou, Greece.
Rock aggregates have been extensively exploited in the construction sector, and the associated engineering features play a critical role in their application. The main aim of this research is to assess the impact of petrographic characteristics on the engineering properties of carbonate rocks. A total of 45 carbonate rock samples from different geological formations within the Salt Range (Western Himalayan Ranges, Pakistan) were subjected to comprehensive petrographic analyses and standard aggregate quality control tests.
View Article and Find Full Text PDFHeliyon
December 2024
Mechanical Engineering Department, Faculty of Engineering, Brawijaya University, MT Haryono167, Malang, 65145, Indonesia.
Industrial organic dyes represent a significant portion of pollutants discharged into the environment, particularly by the textile industry. These compounds pose serious threats to living organisms due to their high toxicity. Various techniques have been explored for the degradation of organic dyes, among which heterogeneous photocatalysis utilising titanium dioxide (TiO) stands out as a promising technology.
View Article and Find Full Text PDFEnviron Monit Assess
December 2024
Wadia Institute of Himalayan Geology, Dehradun, India, 248001.
The Himalayas experiences several cloudburst events due to its varied physiographical, geomorphological, and geological conditions and high rainfall. Uttarakhand is one of the Indian states circumscribed by the Himalayan ranges and has experienced a rise in the number of cloudburst catastrophes in the last few decades. These events cause substantial loss of life and property; however, very few studies have characterized these unpredictable cloudburst-induced flash floods in different regions of Uttarakhand.
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