AI Article Synopsis
- Clonorchiasis, caused by the parasite Clonorchis sinensis, affects around 15 million people predominantly in East Asia.
- Researchers expressed 607 proteins to identify potential antigens for diagnosing the infection, ultimately selecting 18 candidates based on how they reacted with infected human sera.
- Among the tested proteins, CsAg17 showed the best performance with a sensitivity of 77.1% and specificity of 71.2%, making it a promising option for quick diagnostic tests for clonorchiasis.
Article Abstract
Clonorchiasis caused by Clonorchis sinensis is endemic in East Asia; approximately 15 million people have been infected thus far. To diagnose the infection, serodiagnostic tests with excellent functionality should be performed. First, 607 expressed sequence tags encoding polypeptides with a secretory signal were expressed into recombinant proteins using an in vitro translation system. By protein array-based screening using C. sinensis-infected sera, 18 antigen candidate proteins were selected and assayed for cross-reactivity against Opisthorchis viverrini-infected sera. Of the six antigenic proteins selected, four were synthesized on large scale in vitro and evaluated for antigenicity against the flukes-infected human sera using ELISA. CsAg17 antigen showed the highest sensitivity (77.1%) and specificity (71.2%). The sensitivity and specificity of the bacterially produced CsAg17-28GST fusion antigen was similar to those of CsAg17 antigen. CsAg17 antigen can be used to develop point-of-care serodiagnostic tests for clonorchiasis.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7793300 | PMC |
| http://dx.doi.org/10.1371/journal.pntd.0008998 | DOI Listing |
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