The chloroplast genome of the species from Central America has never been reported. With its local use for food flavoring, medicine, and wood, has been of good economic importance. In the present study, the complete chloroplast genome of was assembled via the genome skimming data. As a typical quadripartite structure, the plastome of with 159,906 bp in length includes two inverted repeats (26,554 bp) separated by a small single copy region (18,761 bp) as well as a large single copy region (88,037 bp). This chloroplast genome consists of 131 different genes, including 86 protein coding genes (CDS), eight rRNA genes, and 37 tRNA genes. The maximum likelihood phylogenetic analysis showed that from Central America was closely related to an evergreen species, from East Asia.
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http://dx.doi.org/10.1080/23802359.2020.1715854 | DOI Listing |
STAR Protoc
January 2025
National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:
The plastid-encoded RNA polymerase (PEP) plays an essential role in the transcription of the chloroplast genome. Here, we present a strategy to purify the transcriptionally active protein complex from transplastomic tobacco (Nicotiana tabacum) lines in which one of the PEP core subunits is fused to an epitope tag. We describe experimental procedures for designing transformation constructs for PEP purification, selection, and analysis of transplastomic tobacco plants.
View Article and Find Full Text PDFInt J Mol Sci
January 2025
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China.
s.s. belongs to the Cercidoideae subfamily, located at the base of the Leguminosae family.
View Article and Find Full Text PDFInt J Mol Sci
January 2025
School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.
The Hypericaceae family, comprising nine genera and over seven hundred species, includes plants traditionally used for medicinal purposes. In this study, we performed high-throughput sequencing on three species: , , and , and conducted comparative genomic analyses with related species. The chloroplast genome sizes were 152,654 bp, 122,570 bp, and 137,652 bp, respectively, with an average GC content of 37.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Co-Innovation Center for Sustainable Forestry in Southern China, College of Life Science, Nanjing Forestry University, Nanjing 210037, China.
is a member of the Styracaceae family, which is well-known for its remarkable ornamental and medicinal properties. In this research, we conducted comparative analysis of the chloroplast genomes from four samples of representing . The results demonstrated that the chloroplast genome of four samples ranging from 157,103 bp to 158,357 bp exhibited a typical quadripartite structure, including one large single-copy (LSC) region (90,131 bp to 90,342 bp), one small single-copy (SSC) region (18,467 bp to 18,785 bp), and two inverted repeat regions (IRs) (24,115 bp to 24,261 bp).
View Article and Find Full Text PDFBMC Plant Biol
January 2025
College of Forestry, Southwest Forestry University, Kunming, Yunnan, 650224, China.
Background: Phaius Lour. (Collabieae, Orchidaceae) is a small genus consisting of about 45 species, with highly ornamental and medicinal values. However, the phylogenetic relationship of Phaius among Calanthe s.
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