is a hyperaccumulator on heavy metal with high drought resistance and endurance. Using Illumina next-generation sequencing data, its chloroplast genome is assembled and characterized. The complete chloroplast genome is 159,664 bp in length, consisting of a pair of inverted repeats (IRs) of 26,146 bp each, an 88,854 bp large single-copy (LSC) region and an 18,518 bp small single-copy (SSC) region. It has 137 genes in total, comprising 81 unique protein-coding genes, 32 unique tRNA genes, and 7 rRNA genes. Phylogenetic analysis based on the complete chloroplast genome sequences indicates that is sister to among species with available chloroplast genome sequences.
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http://dx.doi.org/10.1080/23802359.2019.1642153 | DOI Listing |
Proc Natl Acad Sci U S A
February 2025
Molecular Genetics, Institute of Biology, Faculty of Life Sciences, Humboldt Universität zu Berlin, Berlin 10115, Germany.
The chloroplast genome encodes key components of the photosynthetic light reaction machinery as well as the large subunit of the enzyme central for carbon fixation, Ribulose-1,5-bisphosphat-carboxylase/-oxygenase (RuBisCo). Its expression is predominantly regulated posttranscriptionally, with nuclear-encoded RNA-binding proteins (RBPs) playing a key role. Mutants of chloroplast gene expression factors often exhibit impaired chloroplast biogenesis, especially in cold conditions.
View Article and Find Full Text PDFBio Protoc
January 2025
Biochemistry Department, Western University, London, Canada.
Chloroplast genomes present an alternative strategy for large-scale engineering of photosynthetic eukaryotes. Prior to our work, the chloroplast genomes of (204 kb) and (140 kb) had been cloned using bacterial and yeast artificial chromosome (BAC/YAC) libraries, respectively. These methods lack design flexibility as they are reliant upon the random capture of genomic fragments during BAC/YAC library creation; additionally, both demonstrated a low efficiency (≤ 10%) for correct assembly of the genome in yeast.
View Article and Find Full Text PDFPhysiol Plant
January 2025
Laboratory of Biochemistry, Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.
Photosynthetic microalgae are promising green cell factories for the sustainable production of high-value chemicals and biopharmaceuticals. The chloroplast organelle is being developed as a chassis for synthetic biology as it contains its own genome (the plastome) and some interesting advantages, such as high recombinant protein titers and a diverse and dynamic metabolism. However, chloroplast engineering is currently hampered by the lack of standardized cloning tools and Design-Build-Test-Learn workflows to ease genomic and metabolic engineering.
View Article and Find Full Text PDFTree Physiol
January 2025
Institute of Soil and Water Conservation, Northwest A&F University, Yangling 712100, China.
Modulation of stomatal development and movement is a promising approach for creating water-conserving plants. Here, we identified and characterized the PagHCF106 gene of poplar (Populus alba × Populus glandulosa). The PagHCF106 protein localized predominantly to the chloroplast, and the PagHCF106 gene exhibited tissue-specific expression pattern.
View Article and Find Full Text PDFMol Biol Rep
January 2025
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, People's Republic of China.
Background: Paeonia lactiflora Pall., a member of Paeoniaceae family, is a medicinal herb widely used in traditional Chinese medicine. Chloroplasts are multifunctional organelles containing distinct genetic material.
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