Strong loss-of-function or null mutants can sometimes lead to a penetrant early lethality, impairing the study of these genes' function. This is the case for the null mutant, which exhibits 100% penetrant lethality. Here, we describe how we used gene bashing to identify distinct regulatory regions in the locus. This allowed us to generate a null strain that is viable and still displays mutant Y-to-PDA transdifferentiation phenotype. Such strategy can be applied to many other mutants impacting viability.
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| http://dx.doi.org/10.17912/micropub.biology.000339 | DOI Listing |
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- The study focuses on a special group of genes called piRNAs that help female animals stay fertile by stopping bad DNA pieces known as transposons from becoming active.
- Scientists experimented with parts of these genes to learn how they work and found that a protein called Traffic Jam helps control them.
- By looking at the effects of reducing Traffic Jam, the researchers discovered that it not only affects the piRNAs but also helps prevent problems caused by the transposons.
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