Interfere With Bile Acid Enterohepatic Circulation to Regulate Cholesterol Metabolism of Growing-Finishing Pigs via Its Bile Salt Hydrolase Activity.

Microbiota-targeted therapies for hypercholesterolemia get more and more attention and are recognized as an effective strategy for preventing and treating cardiovascular disease. The experiment was conducted to investigate the cholesterol-lowering mechanism of in a pig model. Twelve barrows (38.70 ± 5.33 kg) were randomly allocated to two groups and fed corn-soybean meal diets with either 0% (Con) or 0.1% (Con + LD) for 28 days. fed pigs had lower serum contents of total cholesterol (TC), total bile acids (TBAs), and triglyceride, but higher fecal TC and TBA excretion. treatment increased ileal abundance and bile acid (BA) deconjugation and affected serum and hepatic BA composition. Dietary downregulated the gene expression of ileal apical sodium-dependent bile acid transporter () and ileal bile acid binding protein (), and hepatic farnesoid X receptor (), fibroblast growth factor (), and small heterodimer partner (), but upregulated hepatic high-density lipoprotein receptor (), low-density lipoprotein receptor (), sterol regulatory element binding protein-2 (), and cholesterol-7α hydroxylase () expression. Our results provided evidence that promote ileal BA deconjugation with subsequent fecal TC and TBA extraction by modifying ileal microbiota composition and induce hepatic BA neosynthesis via regulating gut-liver FXR-FGF19 axis.