Evaluation of pretreatments for solubilisation of components and recovery of fermentable monosaccharides from microalgae biomass grown in piggery wastewater.

Chemosphere

Department of Chemical Engineering and Environmental Technology, School of Industrial Engineering, University of Valladolid, Dr Mergelina S/n, 47011, Valladolid, Spain; Institute of Sustainable Processes, University of Valladolid, Dr Mergelina S/n, 47011, Valladolid, Spain. Electronic address:

Published: April 2021

AI Article Synopsis

  • Microalgae-bacteria biomass from wastewater can be transformed into valuable nutrients like carbohydrates, proteins, and lipids through photosynthesis, yet there's limited research on recovering these components effectively.
  • Various pretreatments and enzymatic hydrolysis were tested to enhance the recovery of fermentable sugars from Scenedesmaceae biomass grown in piggery wastewater.
  • Results showed that acid pretreatment maximized carbohydrate solubilization at 98% and monosaccharide recovery at 81%, while physical methods like bead milling improved overall component solubilization but also led to some carbohydrate losses.

Article Abstract

Microalgae-bacteria biomass cultured in wastewater is an interesting renewable material capable of metabolising nutrients from wastes into carbohydrates, proteins, and lipids through photosynthesis. Despite the interest in the valorisation of this biomass to improve the viability of microalgae-based wastewater treatment processes, very scarce research has been devoted to the fractional recovery of its components. This work evaluates the effect of different pretreatments coupled with enzymatic hydrolysis on the solubilisation of biomass components and on the recovery of fermentable monosaccharides (glucose and xylose) from Scenedesmaceae based biomass grown in a thin layer reactor feed with piggery wastewater. Chemical pretreatments generated high concentrations of byproducts, mainly organic acids. No bacterial DNA was found in these pretreated biomasses. The acid pretreatment provided the highest carbohydrate solubilisation (98%) and monosaccharide recovery (81%). Enzymatic hydrolysis coupled with alkaline NaOH 2 M pretreatment achieved almost complete solubilisation of the biomass components, but high carbohydrate losses. Physical pretreatments remarkably increased the solubilisation of the biomass components during the enzymatic hydrolysis step, especially bead milling, which achieved solubilisation yields of 83% of carbohydrates, 43% of proteins, and 60% of lipids. The presence of viable bacteria in these pretreated biomasses could be related to the high carbohydrate losses and the generation of methanol and ethanol in addition to organic acids as byproducts.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chemosphere.2020.129330DOI Listing

Publication Analysis

Top Keywords

enzymatic hydrolysis
12
solubilisation biomass
12
biomass components
12
components recovery
8
recovery fermentable
8
fermentable monosaccharides
8
biomass grown
8
piggery wastewater
8
organic acids
8
pretreated biomasses
8

Similar Publications

Through a quantitative analysis of saltiness perception, favorable enzymatic hydrolysis parameters were confirmed for the preparation of saltiness-enhancing peptide mixtures from . The enzymatic hydrolysate was fractionated into four fractions (F1-F4) by gel chromatography, with F3 exhibiting the strongest saltiness-enhancing effect (22% increase). LC-MS/MS analysis of F3 identified 36 peptides, and their secondary structures and interactions with the TMC4 receptor were examined through circular dichroism spectroscopy and molecular docking.

View Article and Find Full Text PDF

Quercetin (3,3',4',5,7-pentahydroxy flavonoid) is a bioactive flavonoid with significant medicinal properties. Similarly, Rutin (quercetin-3-O-rutinoside or 3',4',5,7-tetrahydroxy-flavone-3-rutinoside) is widely recognized flavonoid, and is converted to quercetin by α-L-rhamnosidase and β-D-glucosidase. In this study, engineered Aspergillus niger co-expressing α-L-rhamnosidase and β-glucosidase was successfully constructed, and engineered strain Rha1-BGL8 was obtained by enzyme activity assay.

View Article and Find Full Text PDF

This study investigated the stabilization mechanism, storage stability, and in vitro digestion characteristics of oil-in-water fish oil emulsions stabilized by β-Lg modified through enzymatic hydrolysis, glycation, and fibrillation. The stabilization mechanism was elucidated by comparing droplet size, ζ-potential, interfacial protein thickness, and microstructure. Results showed that β-Lg modified through these combined processes formed a three-dimensional network, providing superior stabilization, while other modified proteins stabilized emulsions via surface adsorption.

View Article and Find Full Text PDF

Mechanism of differentiated and targeted catalysis in complex lipid system under lipase and lipoxygenase mediation.

Food Chem

December 2024

School of Food Science and Technology, State Key Laboratory of Marine Food Processing & Safety Control, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China. Electronic address:

The regulation of reaction rate differentiation, catalytic precursor differentiation, and end-product differentiation during enzyme-mediated reactions within complex lipid systems is a key area of research in flavor regulation. A multilayer lipid oxidation model, utilizing Plaice bone oil (PBO), lipase, and lipoxygenase, was employed to investigate oxidation differences between various lipids and corresponding flavor formation patterns. Lipase treatment resulted in higher levels of non‑oxygenated volatile compounds and saturated aldehydes, whereas lipoxygenase treatment increased oxygenated compounds, particularly (E)-2-hexenal, 1-penten-3-one, and 2-pentylfuran.

View Article and Find Full Text PDF

Zweifel olefination for C-glycosylation.

Commun Chem

December 2024

Technische Universität Darmstadt, Clemens-Schöpf-Institut für Organische Chemie und Biochemie, Peter-Grünberg-Straße 4, 64287, Darmstadt, Germany.

C-glycosides are significant in medicinal chemistry due to their resistance to enzymatic hydrolysis, making them more stable and bioavailable compared to O-glycosides. Their unique structure also offers potential for developing drugs with improved therapeutic properties, particularly in treating diseases like diabetes and cancer. The main challenge in synthesizing C-glycosides lies in forming the carbon-carbon bond between the sugar and aglycone efficiently, while controlling the stereochemistry and minimizing side reactions.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!

A PHP Error was encountered

Severity: Notice

Message: fwrite(): Write of 34 bytes failed with errno=28 No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 272

Backtrace:

A PHP Error was encountered

Severity: Warning

Message: session_write_close(): Failed to write session data using user defined save handler. (session.save_path: /var/lib/php/sessions)

Filename: Unknown

Line Number: 0

Backtrace: