Spheroplasts, poorly known but clinically relevant particles of urinary sediment.

Background: In two patients under treatment with various antibiotics, spheroplasts were detected with an automated urine sediment analyzer.

Methods: Urinalysis was performed by an AutionMAX AX 4030-sediMAX platform.

Results: Spheroplasts can be easily misclassified as yeasts or erythrocytes, but when automated urine sediment analyzers are used by well-trained, and experienced operators they can be correctly identified and classified.

Conclusion: Appropriate training of urine laboratory professionals in spheroplast detection and association with UTI, together with timely communication with the microbiologist and caring clinician, will provide prompt targeted treatment.