Relative Deficiency in Albumin Methionine Content is Associated With Decreased Antioxidant Capacity of Equine Plasma.

Relative to other species, horses seem particularly susceptible to oxidative stress. Plasma albumin plays an important role in preventing oxidative damage, in part due to its methionine (MET) content. Equine albumin is highly unusual in that it contains no MET residues. Whether or not this causes deficient antioxidant capacity in equine plasma relative to that of other species has not yet been explored. The objective of this study was to compare the redox status of equine (no MET) to that of bovine (moderate amount of MET) plasma. Plasma was collected from healthy, nonpregnant Quarter Horse mares (n = 10) and adult, healthy, nonpregnant, dried Holstein cows (n = 15). Measures of total antioxidant capacity and oxidative stress were assessed for each plasma sample using multiple commercially available assays: total antioxidant capacity, thiol detection, thiobarbituric acid reactive substances, and advanced oxidation protein products. Plasma from horses had significantly (P < .05) lower thiol content and thiobarbituric and reactive substances and higher advanced oxidation protein products than plasma from cattle. A difference in total antioxidant capacity was not observed; however, our study was underpowered to establish a meaningful comparison. Based on these findings, the lack of MET in equine albumin appears to translate to a lower antioxidant capacity of equine plasma. Our findings are consistent with previous reports in other species that identify MET as having an important role in the antioxidant capacity of albumin. Our results also highlight the complex system of antioxidant defenses in plasma that counteract the harmful effects of oxidants.