AI Article Synopsis
- Removal of biofilms is crucial in both environmental and medical contexts, making research on effective enzyme combinations for disrupting them very important.
- The study focused on a specific enzyme, protease from bovine pancreas type-I (PtI), and its anti-biofilm effects, both alone and in combination with another enzyme, α-amylase (αA).
- Results showed that PtI alone had strong biofilm inhibition against multiple bacteria, while the combination of PtI and αA further enhanced these effects, suggesting their potential utility in treating biofilm-related infections, especially those caused by MRSA.
Article Abstract
Removal of biofilms is extremely pivotal in environmental and medicinal fields. Therefore, reporting the new-enzymes and their combinations for dispersal of infectious biofilms can be extremely critical. Herein, for the first time, we accessed the enzyme "protease from bovine pancreas type-I (PtI)" for anti-biofilm properties. We further investigated the anti-biofilm potential of PtI in combination with α-amylase from . (αA). PtI showed a very significant biofilm inhibition effect (86.5%, 88.4%, and 67%) and biofilm prevention effect (66%, 64%, and 70%), against the , , and MRSA, respectively. However, the new enzyme combination (-PtI+αA) exhibited biofilm inhibition effect (78%, 90%, and 93%) and a biofilm prevention effect (44%, 51%, and 77%) against , , and MRSA, respectively. The studied enzymes were found not to be anti-bacterial against the , , and MRSA. In summary, the PtI exhibited significant anti-biofilm effects against , MRSA, and . -PtI+αA exhibited enhancement of the anti-biofilm effects against and MRSA biofilms. Therefore, this study revealed that this -PtI+αA enzymatic system can be extremely vital for the treatment of biofilm complications resulting from , , and MRSA.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7766206 | PMC |
| http://dx.doi.org/10.3390/polym12123032 | DOI Listing |
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